Zhang Yang, Pei Guangsheng, Chen Lei, Zhang Weiwen
a Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology , Tianjin University , Tianjin , PR China.
b Key Laboratory of Systems Bioengineering (Ministry of Education) , Tianjin University , Tianjin , PR China.
Biofouling. 2016 Aug;32(7):725-36. doi: 10.1080/08927014.2016.1193166.
In this study, a comparative metabolomics approach combining gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) was applied first between planktonic cells and biofilms and then between pure cultures and biofilms of Desulfovibrio vulgaris. The results revealed that the overall metabolic level of the biofilm cells was down-regulated, especially for metabolites related to the central carbon metabolism, compared to the planktonic cells and the pure culture of D. vulgaris. In addition, pathway enrichment analysis of the 58 metabolites identified by GC-MS showed that fatty acid biosynthesis in the biofilm cells was up-regulated, suggesting that fatty acids may be important for the formation, maintenance and function of D. vulgaris biofilm. This study offers a valuable perspective on the metabolic dynamics of the D. vulgaris biofilm.
在本研究中，首先应用气相色谱 - 质谱联用（GC - MS）和液相色谱 - 质谱联用（LC - MS）的比较代谢组学方法，对浮游细胞与生物膜之间，以及普通脱硫弧菌的纯培养物与生物膜之间进行了比较。结果表明，与浮游细胞和普通脱硫弧菌的纯培养物相比，生物膜细胞的整体代谢水平下调，特别是与中心碳代谢相关的代谢物。此外，对通过GC - MS鉴定出的58种代谢物进行的通路富集分析表明，生物膜细胞中的脂肪酸生物合成上调，这表明脂肪酸可能对普通脱硫弧菌生物膜的形成、维持和功能很重要。本研究为普通脱硫弧菌生物膜的代谢动态提供了有价值的观点。
