Higson F K, Focht D D
Department of Soil and Environmental Sciences, University of California, Riverside 92521.
Appl Environ Microbiol. 1989 Apr;55(4):946-52. doi: 10.1128/aem.55.4.946-952.1989.
Isolates able to grow on 3- or 4-hydroxybiphenyl (HB) as the sole carbon source were obtained by enrichment culture. The 3-HB degrader Pseudomonas sp. strain FH12 used an NADPH-dependent monooxygenase restricted to 3- and 3,3'-HBs to introduce an ortho-hydroxyl. The 4-HB degrader Pseudomonas sp. strain FH23 used either a mono- or dioxygenase to generate a 2,3-diphenolic substitution pattern which allowed meta-fission of the aromatic ring. By using 3-chlorocatechol to inhibit catechol dioxygenase activity, it was found that 2- and 3-HBs were converted by FH23 to 2,3-HB, whereas biphenyl and 4-HB were attacked by dioxygenation. 4-HB was metabolized to 2,3,4'-trihydroxybiphenyl. Neither organism attacked chlorinated HBs. The degradation of 3- and 4-HBs by these strains is therefore analogous to the metabolism of biphenyl, 2-HB, and naphthalene in the requirement for 2,3-catechol formation.
通过富集培养获得了能够以3-或4-羟基联苯（HB）作为唯一碳源生长的菌株。3-HB降解菌假单胞菌属菌株FH12使用一种依赖NADPH的单加氧酶，该酶仅限于3-和3,3'-HBs以引入邻位羟基。4-HB降解菌假单胞菌属菌株FH23使用单加氧酶或双加氧酶来生成2,3-二酚取代模式，从而允许芳环的间位裂解。通过使用3-氯儿茶酚抑制儿茶酚双加氧酶活性，发现FH23将2-和3-HBs转化为2,3-HB，而联苯和4-HB则通过双加氧作用被攻击。4-HB被代谢为2,3,4'-三羟基联苯。两种菌株都不攻击氯化HBs。因此，这些菌株对3-和4-HBs的降解类似于对联苯、2-HB和萘的代谢，都需要形成2,3-儿茶酚。
