Lange J, Hammer E, Specht M, Francke W, Schauer F
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Appl Microbiol Biotechnol. 1998 Sep;50(3):364-8. doi: 10.1007/s002530051305.
Cells of the yeast strain Debaryomyces vanrijiae SBUG 770, grown with glucose, converted biphenyl to 4-hydroxybiphenyl as the major metabolite. In addition, 2-hydroxybiphenyl was formed in minor amounts. No further degradation of these substances was detected. However, these monohydroxylated derivatives were oxidised by alkane-grown cells in the presence of the co-metabolic substrate, tetradecane. Under these conditions 2-hydroxybiphenyl was oxidised to 2,5-dihydroxybiphenyl, and 4-hydroxybiphenyl was rapidly metabolised by formation of two major metabolites. One was identified as 3,4-dihydroxybiphenyl. Characterisation of the second product as 4-phenylmuconolactone points to a further metabolism of the initially formed dihydroxylated biphenyl via ortho-ring fission.
在以葡萄糖为生长底物的条件下，季也蒙毕赤酵母菌株Debaryomyces vanrijiae SBUG 770的细胞将联苯转化为主要代谢产物4-羟基联苯。此外，还生成了少量的2-羟基联苯。未检测到这些物质的进一步降解。然而，在共代谢底物十四烷存在的情况下，这些单羟基化衍生物被以烷烃为生长底物的细胞氧化。在这些条件下，2-羟基联苯被氧化为2,5-二羟基联苯，4-羟基联苯通过形成两种主要代谢产物而迅速代谢。其中一种被鉴定为3,4-二羟基联苯。将第二种产物鉴定为4-苯基粘康酸内酯表明最初形成的二羟基化联苯通过邻位环裂解进一步代谢。
