Jiang Z, Tao J-H, Zuo T, Li X-M, Wang G-S, Fang X, Xu X-L, Li X-P
a Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital , Anhui Medical University , Hefei , China.
b Department of Rheumatology and Immunology, Affiliated Huai'an First People's Hospital , Nanjing Medical University , Nanjing , China.
Scand J Rheumatol. 2017 Mar;46(2):122-129. doi: 10.3109/03009742.2016.1167950. Epub 2016 Jun 16.
To explore the correlation between microRNA (miR)-200c and the severity of interstitial lung disease (ILD) associated with connective tissue diseases (CTDs).
We recruited 218 patients with CTDs who were evaluated with high-resolution computed tomography (HRCT) and the pulmonary function test (PFT). Peripheral blood mononuclear cells (PBMCs) were acquired from 23 patients with systemic sclerosis (SSc), 29 with dermatomyositis/polymyositis (DM/PM), 30 with primary Sjögren's syndrome (pSS), 47 with rheumatoid arthritis (RA), and 23 normal controls to detect the expression level of miR-200c by quantitative reverse transcription polymerase chain reaction (QRT-PCR). miR-200c levels were compared among the different disease groups, between the group with ILD (CTD+ILD) and the group without ILD (CTD-ILD), and between mild and severe ILD groups. Forced vital capacity (FVC) and forced expiratory volume in 1 s (FEV1) were compared among the different CTD groups and the different CTD+ILD groups.
The miR-200c level in the SSc group was significantly higher than in the DM/PM, pSS, and RA groups, and the levels in the DM/PM and pSS groups were significantly higher than in the RA group. The level of miR-200c in the CTD+ILD group was significantly higher than in the CTD-ILD group, and the level in the severe ILD group was significantly higher than in the mild ILD group. FVC and FEV1 were significantly different among the different CTD groups, and among the different CTD+ILD groups. There was a negative correlation between the level of miR-200c and FVC and FEV1.
The level of miR-200c was positively correlated with the severity of ILD, and miR-200c in PBMCs could be a biomarker of the severity of ILD in CTDs.
探讨微小RNA（miR）-200c与结缔组织病（CTD）相关间质性肺病（ILD）严重程度之间的相关性。
我们招募了218例CTD患者，对其进行高分辨率计算机断层扫描（HRCT）和肺功能测试（PFT）评估。从23例系统性硬化症（SSc）患者、29例皮肌炎/多肌炎（DM/PM）患者、30例原发性干燥综合征（pSS）患者、47例类风湿关节炎（RA）患者以及23名正常对照者中获取外周血单个核细胞（PBMC），采用定量逆转录聚合酶链反应（QRT-PCR）检测miR-200c的表达水平。比较不同疾病组之间、有ILD组（CTD+ILD）和无ILD组（CTD-ILD）之间以及轻度和重度ILD组之间的miR-200c水平。比较不同CTD组以及不同CTD+ILD组之间的用力肺活量（FVC）和第1秒用力呼气量（FEV1）。
SSc组的miR-200c水平显著高于DM/PM组、pSS组和RA组，DM/PM组和pSS组的水平显著高于RA组。CTD+ILD组的miR-200c水平显著高于CTD-ILD组，重度ILD组的水平显著高于轻度ILD组。不同CTD组之间以及不同CTD+ILD组之间的FVC和FEV1存在显著差异。miR-200c水平与FVC和FEV1呈负相关。
miR-200c水平与ILD严重程度呈正相关，PBMC中的miR-200c可能是CTD中ILD严重程度的生物标志物。
