Ishemgulova Aygul, Kraeva Natalya, Faktorova Drahomira, Podesvova Lucie, Lukes Julius, Yurchenko Vyacheslav
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic;
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic;
Folia Parasitol (Praha). 2016 May 18;63:2016.016. doi: 10.14411/fp.2016.016.
In our previous work we established a T7 polymerase-driven Tetracycline-inducible protein expression system in Leishmania mexicana (Biagi, 1953). We used this system to analyse gene expression profiles during development of L. mexicana in procyclic and metacyclic promastigotes and amastigotes. The transcription of the gene of interest and the T7 polymerase genes was significantly reduced upon cell differentiation. This regulation is not locus-specific. It depends on untranslated regions flanking open reading frames of the genes analysed. In this paper, we report that the previously established conventional inducible protein expression system may not be suitable for studies on differentiation of species of Leishmania Ross, 1903 and protein expression systems might have certain limitations.
在我们之前的工作中，我们在墨西哥利什曼原虫（Biagi，1953）中建立了一种由T7聚合酶驱动的四环素诱导型蛋白质表达系统。我们使用该系统分析了墨西哥利什曼原虫在前鞭毛体和后鞭毛体以及无鞭毛体发育过程中的基因表达谱。在细胞分化时，目的基因和T7聚合酶基因的转录显著降低。这种调控不是位点特异性的。它取决于所分析基因开放阅读框两侧的非翻译区。在本文中，我们报告先前建立的传统诱导型蛋白质表达系统可能不适用于对1903年罗斯利什曼原虫物种分化的研究，并且蛋白质表达系统可能存在某些局限性。
