The First Non-LRV RNA Virus in .

In this work, we describe the first -infecting leishbunyavirus-the first virus other than (LRV) found in trypanosomatid parasites. Its host is , a human pathogen causing infections with a wide range of manifestations from asymptomatic to severe visceral disease. This virus (LBV1) possesses many characteristic features of leishbunyaviruses, such as tripartite organization of its RNA genome, with ORFs encoding RNA-dependent RNA polymerase, surface glycoprotein, and nucleoprotein on L, M, and S segments, respectively. Our phylogenetic analyses suggest that LBV1 originated from leishbunyaviruses of monoxenous trypanosomatids and, probably, is a result of genomic re-assortment. The LBV1 facilitates parasites' infectivity in vitro in primary murine macrophages model. The discovery of a virus in poses the question of whether it influences pathogenicity of this parasite in vivo, similarly to the LRV in other species.