Gruszewska Ewa, Cylwik Bogdan, Gudowska Monika, Kedra Boguslaw, Szmitkowski Maciej, Chrostek Lech
Department of Biochemical Diagnostics, Medical University of Bialystok, Bialystok, Poland
Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland.
Ann Clin Lab Sci. 2016 May;46(3):286-90.
The aim of this study was to assess the alterations in transferrin sialylation during primary pancreatic cancer, and compare these with CA19-9. Serum samples from 44 patients with pancreatic cancer and 25 healthy subjects were analyzed by capillary electrophoresis (CE) technology on a MINICAP electrophoresis system. The mean concentration of tetrasialotransferrin was significantly higher while that of pentasialotransferrin was significantly lower in cancer patients than in the controls. However, there were no significant differences in the concentrations of disialotransferrin and trisialotranferrin in pancreatic cancers. Also, total transferrin concentration did not differ between pancreatic cancer patients and control group. Neither tumor location nor TNM classification affected the concentrations of CA19-9 and transferrin isoforms with the exception of trisialotransferrin which was significantly higher in patients free of distant metastasis than in those with distant ones. The ratio of tetrasialotransferrin to pentasialotransferrin in pancreatic cancer patients was significantly higher than that in the controls. We conclude that the alterations in transferrin sialylation occur in patients with pancreatic cancer. Tetrasialotransferrin was more strongly expressed than pentasialotransferrin. Moreover, the data suggests that the alterations in transferrin glycosylation/sialylation in pancreatic cancer differ from the alterations in some other cancers.
本研究旨在评估原发性胰腺癌患者转铁蛋白唾液酸化的变化，并将其与CA19-9进行比较。采用MINICAP电泳系统，通过毛细管电泳（CE）技术对44例胰腺癌患者和25例健康受试者的血清样本进行分析。结果显示，与对照组相比，癌症患者血清中四唾液酸转铁蛋白的平均浓度显著升高，而五唾液酸转铁蛋白的平均浓度显著降低。然而，胰腺癌患者双唾液酸转铁蛋白和三唾液酸转铁蛋白的浓度与对照组无显著差异。此外，胰腺癌患者与对照组的总转铁蛋白浓度无差异。除三唾液酸转铁蛋白在无远处转移患者中的浓度显著高于有远处转移患者外，肿瘤位置和TNM分期均不影响CA19-9和转铁蛋白异构体的浓度。胰腺癌患者四唾液酸转铁蛋白与五唾液酸转铁蛋白的比值显著高于对照组。我们得出结论，胰腺癌患者存在转铁蛋白唾液酸化的变化，四唾液酸转铁蛋白的表达强于五唾液酸转铁蛋白。此外，数据表明胰腺癌中转铁蛋白糖基化/唾液酸化的变化与其他一些癌症不同。
