Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
Carbohydr Polym. 2016 Oct 5;150:408-18. doi: 10.1016/j.carbpol.2016.03.088. Epub 2016 Mar 31.
Alginate-based membranes containing hyaluronic acid (HA) were manufactured by freeze-drying calcium-reticulated hydrogels. The study of the distribution of the two macromolecules within the hydrogel enabled to highlight a polymer demixing mechanism that tends to segregate HA in the external parts of the constructs. Resistance and pliability of the membranes were tuned, while release and degradation studies enabled to quantify the diffusion of both polysaccharides in physiological solution and to measure the viable lifetime of the membranes. Biological studies in vitro proved that the liquid extracts from the HA-containing membranes stimulate wound healing and that fibroblasts are able to colonize the membranes. Overall, such novel alginate-HA membranes represent a promising solution for several medical needs, in particular for wound treatment, giving the possibility to provide an in situ administration of HA from a resorbable device.
通过冷冻干燥钙离子交联水凝胶制备了含有透明质酸（HA）的海藻酸盐基膜。研究两种高分子在水凝胶中的分布，突出了一种聚合物分相机制，该机制倾向于将 HA 分离到构建体的外部。通过调节膜的阻力和柔韧性，同时进行释放和降解研究，定量研究了两种多糖在生理溶液中的扩散，并测量了膜的有效寿命。体外生物学研究证明，含有 HA 的膜的液体提取物能刺激伤口愈合，成纤维细胞能够定植于膜中。总之，这种新型的海藻酸盐-HA 膜为许多医疗需求提供了一种很有前途的解决方案，特别是在伤口治疗方面，有可能通过可吸收装置提供 HA 的局部给药。
