• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

通过改进牛人工授精中使用的冷链设备来保持精液质量。

Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination.

机构信息

Intellectual Ventures Laboratory, Bellevue, Washington, United States of America.

出版信息

Sci Rep. 2016 Jun 17;6:28108. doi: 10.1038/srep28108.

DOI:10.1038/srep28108
PMID:27313137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4911568/
Abstract

Artificial insemination of dairy cattle is a common practice in the developing world that can improve farmer incomes and food security. Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and stored is crucial to the success of this process. Here we describe simple technological improvements to protect semen from inadvertent thermal fluctuations that occur when users mishandle semen using standard equipment. We show that when frozen semen is mishandled, characteristics of semen biology associated with fertility are negatively affected. We describe several design modifications and results from thermal performance tests of several improved prototypes. Finally, we compare semen that has been mishandled in standard and improved equipment. The data suggest that our canister improvements can better maintain characteristics of semen biology that correlate with fertility when it is mishandled.

摘要

奶牛人工授精是发展中国家的一种常见做法,它可以提高农民的收入和粮食安全。在这个过程中,保持冷冻精液在处理、运输和储存过程中的受精能力至关重要。在这里,我们描述了一些简单的技术改进,以保护精液免受用户在使用标准设备时无意中造成的热波动的影响。我们发现,当冷冻精液被不当处理时,与生育能力相关的精液生物学特性会受到负面影响。我们描述了几种设计改进,并展示了几种改进原型的热性能测试结果。最后,我们比较了在标准设备和改进设备中不当处理的精液。这些数据表明,我们的罐式改进可以更好地保持在处理不当的情况下与生育能力相关的精液生物学特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/e7c169f4e09a/srep28108-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/b9d65b288807/srep28108-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/5ac4ddbec51a/srep28108-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/bbf58ec40049/srep28108-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/45a74c49fcf0/srep28108-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/da8ce3ed8416/srep28108-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/e7c169f4e09a/srep28108-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/b9d65b288807/srep28108-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/5ac4ddbec51a/srep28108-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/bbf58ec40049/srep28108-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/45a74c49fcf0/srep28108-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/da8ce3ed8416/srep28108-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed5/4911568/e7c169f4e09a/srep28108-f6.jpg

相似文献

1
Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination.通过改进牛人工授精中使用的冷链设备来保持精液质量。
Sci Rep. 2016 Jun 17;6:28108. doi: 10.1038/srep28108.
2
Pregnancy and conception rate after two intravaginal inseminations with dog semen frozen either with 5% glycerol or 5% ethylene glycol.用 5%甘油或 5%乙二醇冷冻的狗精液进行两次阴道内授精后的妊娠和受孕率。
Anim Reprod Sci. 2010 Mar;118(1):94-7. doi: 10.1016/j.anireprosci.2009.06.007.
3
Fertility of dairy cattle after artificial insemination with semen frozen in a lipoprotein diluent.用脂蛋白稀释剂冷冻精液对奶牛进行人工授精后的繁殖力。
J Reprod Fertil. 1977 Sep;51(1):175-7. doi: 10.1530/jrf.0.0510175.
4
Effect of glycerol on the viability and fertility of cooled bovine semen.甘油对冷冻牛精液活力和受精能力的影响。
Theriogenology. 2015 Jan 1;83(1):107-13. doi: 10.1016/j.theriogenology.2014.08.009.
5
Reproductive aspects and storage of semen in camelidae.骆驼科动物精液的生殖特性与保存
Anim Reprod Sci. 2000 Aug 18;62(1-3):173-93. doi: 10.1016/s0378-4320(00)00158-5.
6
Successful chilling of red-legged partridge (Alectoris rufa) sperm for use in artificial insemination.成功冷却红腿鹧鸪(Alectoris rufa)精子用于人工授精。
Poult Sci. 2017 Sep 1;96(11):4068-4074. doi: 10.3382/ps/pex176.
7
A comparison of semen diluents on the in vitro and in vivo fertility of liquid bull semen.公牛精液稀释剂对液态公牛精液体外和体内受精能力的比较。
J Dairy Sci. 2017 Feb;100(2):1541-1554. doi: 10.3168/jds.2016-11646. Epub 2016 Nov 23.
8
Semen preservation and artificial insemination in domesticated South American camelids.家畜化南美驼马的精液保存与人工授精。
Anim Reprod Sci. 2013 Jan 10;136(3):157-63. doi: 10.1016/j.anireprosci.2012.10.005. Epub 2012 Oct 24.
9
Fertility after insemination with frozen-thawed sperm using N-methylacetamide extender on the Combatiente Español avian breed.使用 N-甲基乙酰胺作为解冻液,对西班牙战斗鸡品种进行冻精人工授精后的繁殖力。
Anim Reprod Sci. 2019 Sep;208:106111. doi: 10.1016/j.anireprosci.2019.106111. Epub 2019 Jun 28.
10
[Chilled and frozen semen: the animal experience].
Gynecol Obstet Fertil. 2004 Oct;32(10):887-93. doi: 10.1016/j.gyobfe.2004.09.003.

引用本文的文献

1
Freezing Hu ram semen: influence of different penetrating cryoprotectants and egg yolk level on the post-thaw quality of sperm.冷冻胡枝子精液:不同穿透性冷冻保护剂和蛋黄水平对解冻后精子质量的影响。
Anim Biosci. 2024 Sep;37(9):1548-1557. doi: 10.5713/ab.24.0167. Epub 2024 Jun 25.
2
Using on-demand dry ice production as an alternative cryogenic cold chain for bovine artificial insemination outreach in low-resource settings.在资源匮乏地区,利用按需生产干冰作为牛人工授精推广的替代低温冷链。
Transl Anim Sci. 2020 Feb 3;4(2):txaa012. doi: 10.1093/tas/txaa012. eCollection 2020 Apr.
3
Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation.

本文引用的文献

1
Storage of human biospecimens: selection of the optimal storage temperature.人类生物样本的储存:最佳储存温度的选择
Biopreserv Biobank. 2014 Jun;12(3):165-75. doi: 10.1089/bio.2013.0084. Epub 2014 Jun 11.
2
Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos.储存和处理条件对卵母细胞及胚胎玻璃化转变和潜在失透的影响。
Theriogenology. 2014 Aug;82(3):373-8. doi: 10.1016/j.theriogenology.2014.04.003. Epub 2014 Apr 15.
3
A perspective on the impact of reproductive technologies on food production in Africa.
通过油包水电穿孔法将质粒和蛋白质导入牛和猪细胞。
J Vet Med Sci. 2020 Jan 10;82(1):14-22. doi: 10.1292/jvms.19-0475. Epub 2019 Nov 27.
对生殖技术对非洲粮食生产的影响的看法。
Adv Exp Med Biol. 2014;752:199-211. doi: 10.1007/978-1-4614-8887-3_10.
4
Fertilization: a sperm's journey to and interaction with the oocyte.受精:精子的旅程及其与卵子的相互作用。
J Clin Invest. 2010 Apr;120(4):984-94. doi: 10.1172/JCI41585. Epub 2010 Apr 1.
5
Improved methodology for the detection and quantification of the acrosome reaction in mouse spermatozoa.用于检测和定量小鼠精子顶体反应的改进方法。
Histol Histopathol. 2009 Aug;24(8):999-1007. doi: 10.14670/HH-24.999.
6
Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes.牛精子质膜、顶体膜和线粒体膜同步荧光评估的实用技术。
Reprod Domest Anim. 2007 Oct;42(5):479-88. doi: 10.1111/j.1439-0531.2006.00810.x.
7
Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test.通过使用荧光花生凝集素结合低渗肿胀试验评估新鲜和冷冻保存标本中的顶体状态和精子活力。
Int Braz J Urol. 2007 May-Jun;33(3):364-74; discussion 375-6. doi: 10.1590/s1677-55382007000300009.
8
Can we increase the estimated value of semen assessment?我们能否提高精液评估的估计值?
Reprod Domest Anim. 2006 Oct;41 Suppl 2:2-10. doi: 10.1111/j.1439-0531.2006.00764.x.
9
Fertilizing capacity of bull spermatozoa after freezing at 79 degrees C.79摄氏度冷冻后公牛精子的受精能力
Nature. 1952 Apr 12;169(4302):626-7. doi: 10.1038/169626b0.
10
Laboratory semen assessment and prediction of fertility: still utopia?实验室精液评估与生育能力预测:仍为乌托邦式的空想?
Reprod Domest Anim. 2003 Aug;38(4):312-8. doi: 10.1046/j.1439-0531.2003.00436.x.