多个时间点的基因表达分析确定了神经再生的关键基因。
Gene expression analysis at multiple time-points identifies key genes for nerve regeneration.
作者信息
Pan Bin, Liu Yi, Yan Jia-Yin, Wang Yao, Yao Xue, Zhou Heng-Xing, Lu Lu, Kong Xiao-Hong, Feng Shi-Qing
机构信息
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
School of Medicine, Nankai University, Nankai District, Tianjin, PR China.
出版信息
Muscle Nerve. 2017 Mar;55(3):373-383. doi: 10.1002/mus.25225. Epub 2016 Nov 23.
INTRODUCTION
The purpose of this study was to provide a comprehensive understanding of gene expression during Wallerian degeneration and axon regeneration after peripheral nerve injury.
METHODS
A microarray was used to detect gene expression in the distal nerve 0, 3, 7, and 14 days after sciatic nerve crush. Bioinformatic analysis was used to predict function of the differentially expressed mRNAs. Microarray results and the key pathways were validated by quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
Differentially expressed mRNAs at different time-points (3, 7, and 14 days) after injury were identified and compared with a control group (0 day). Nine general trends of changes in gene expression were identified. Key signal pathways and 9 biological processes closely associated with nerve regeneration were identified and verified.
CONCLUSIONS
Differentially expressed genes and biological processes and pathways associated with axonal regeneration may elucidate the molecular-biological mechanisms underlying peripheral nerve regeneration. Muscle Nerve 55: 373-383, 2017.
引言
本研究的目的是全面了解周围神经损伤后沃勒变性和轴突再生过程中的基因表达情况。
方法
使用微阵列检测坐骨神经挤压后0、3、7和14天远端神经中的基因表达。利用生物信息学分析预测差异表达mRNA的功能。通过定量实时聚合酶链反应（qRT-PCR）验证微阵列结果和关键途径。
结果
确定了损伤后不同时间点（3、7和14天）差异表达的mRNA，并与对照组（0天）进行比较。确定了基因表达变化的九个总体趋势。确定并验证了与神经再生密切相关的关键信号通路和九个生物学过程。
结论
与轴突再生相关的差异表达基因、生物学过程和途径可能阐明周围神经再生的分子生物学机制。《肌肉与神经》55: 373 - 383, 2017年。
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