Knight G J, Palomaki G E, Lea D H, Haddow J E
Foundation for Blood Research, Scarborough, ME 04074.
Clin Chem. 1989 Jun;35(6):1036-9.
We describe a polyclonal-antiserum-based 125I-radioimmunoassay for cotinine that is suitable for measuring nonsmokers' passive exposure to tobacco smoke in the environment. The standard curve ranged from 0.25 to 12.0 micrograms/L, with an estimated lower limit of sensitivity of 0.2 microgram/L (95% B/Bo = 0.2 microgram/L; 50% B/Bo = 4.0 micrograms/L). The median within-assay CVs for patients' samples with cotinine values from 0.4 to 1.3, 1.4 to 2.4, 2.5 to 4.6, and 4.7 to 15.6 micrograms/L were 13.9%, 7.2%, 5.1%, and 5.7%, respectively. Between-assay CVs for two quality-control sera with average values of 1.53 and 3.68 micrograms/L were 14.3% and 7.8%, respectively. Analytical recoveries of cotinine from smokers' sera diluted in zero calibrant ranged from 91% to 116%. Cotinine values determined on 79 paired sera and urines from nonsmokers showed significant correlation with self-reported exposure to environmental tobacco smoke (r = 0.49, P less than 0.001 for sera; r = 0.57, P less than 0.001 for urine). The log of the values for serum and urine cotinine were also significantly correlated (r = 0.85, P less than 0.001). Evidently, polyclonal antiserum can be used to develop a cotinine assay for measuring exposure to environmental tobacco smoke that compares well with that described for monoclonal-based assays.
我们描述了一种基于多克隆抗血清的可替宁125I放射免疫分析法，该方法适用于测量非吸烟者在环境中被动接触烟草烟雾的情况。标准曲线范围为0.25至12.0微克/升，估计灵敏度下限为0.2微克/升（95% B/Bo = 0.2微克/升；50% B/Bo = 4.0微克/升）。可替宁值在0.4至1.3、1.4至2.4、2.5至4.6以及4.7至15.6微克/升的患者样本的批内CV中位数分别为13.9%、7.2%、5.1%和5.7%。两种平均浓度为1.53和3.68微克/升的质控血清的批间CV分别为14.3%和7.8%。在零校准剂中稀释的吸烟者血清中可替宁的分析回收率为91%至116%。对79对非吸烟者的血清和尿液进行检测，结果显示可替宁值与自我报告的环境烟草烟雾暴露量显著相关（血清：r = 0.49，P < 0.001；尿液：r = 0.57，P < 0.001）。血清和尿液中可替宁值的对数也显著相关（r = 0.85, P < 0.001）。显然，多克隆抗血清可用于开发一种可替宁检测方法，以测量环境烟草烟雾暴露量，该方法与基于单克隆抗体的检测方法相比效果良好。
