Jiang Shuting, Chen Weihao, Zhang Yimin, Zhang Yujuan, Chen Ailian, Dai Qiufu, Lin Shujun, Lin Hanyu
Medical College, Jinan University, 601 Huangpu West Avenue, Guangzhou, Guangdong 510632, China.
Xiangzhou District People's Hospital, 178 Lanpu Road, Xiangzhou District, Zhuhai, Guangdong 519070, China.
Evid Based Complement Alternat Med. 2016;2016:2047412. doi: 10.1155/2016/2047412. Epub 2016 May 25.
Purpose. To investigate whether acupuncture induced the proliferation and differentiation of endogenous neural stem cells (NSCs) in a rat model of traumatic brain injury (TBI). Methods. 104 Sprague-Dawley rats were randomly divided into normal, model, and acupuncture groups. Each group was subdivided into three-day (3 d), seven-day (7 d), and fourteen-day (14 d) groups. The rat TBI model was established using Feeney's freefall epidural impact method. The rats in the acupuncture group were treated at acupoints (Baihui, Shuigou, Fengfu, Yamen, and bilateral Hegu). The normal and model groups did not receive acupuncture. The establishment of the rat TBI model and the therapeutic effect of acupuncture were assessed using neurobehavioral scoring and hematoxylin-eosin staining. The proliferation and differentiation of NSCs in TBI rats were analyzed using immunofluorescence microscopy. Results. The levels of nestin-expressing cells and bromodeoxyuridine/glial fibrillary acidic protein- (BrdU/GFAP-) and BrdU/S100 calcium-binding protein B-positive and BrdU/microtubule-associated protein 2- and BrdU/galactocerebrosidase-positive cells were more significantly increased at various time points in the acupuncture group than in the model group (P < 0.01), except for a decreased level of BrdU/GFAP-positive cells at 7 d and 14 d. Conclusion. Acupuncture induced the proliferation and differentiation of NSCs, thereby promoting neural repair in the TBI rats.
目的。探讨针刺是否能诱导创伤性脑损伤（TBI）大鼠模型中内源性神经干细胞（NSCs）的增殖和分化。方法。104只Sprague-Dawley大鼠随机分为正常组、模型组和针刺组。每组再分为3天（3 d）、7天（7 d）和14天（14 d）亚组。采用Feeney自由落体硬膜外撞击法建立大鼠TBI模型。针刺组大鼠在穴位（百会、水沟、风府、哑门和双侧合谷）进行治疗。正常组和模型组不接受针刺。采用神经行为评分和苏木精-伊红染色评估大鼠TBI模型的建立及针刺的治疗效果。采用免疫荧光显微镜分析TBI大鼠中NSCs的增殖和分化。结果。除7 d和14 d时BrdU/GFAP阳性细胞水平降低外，针刺组各时间点表达巢蛋白的细胞以及溴脱氧尿苷/胶质纤维酸性蛋白（BrdU/GFAP）、BrdU/S100钙结合蛋白B阳性、BrdU/微管相关蛋白2和BrdU/半乳糖脑苷脂阳性细胞的水平均比模型组显著升高（P < 0.01）。结论。针刺诱导了NSCs的增殖和分化，从而促进了TBI大鼠的神经修复。
