Zhao Xiaojuan, Zhou Yun, Chen Y U, Yu Feng
Department of Gynecology and Obstetrics, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, Jiangsu 214000, P.R. China.
Oncol Lett. 2016 Jun;11(6):4245-4251. doi: 10.3892/ol.2016.4527. Epub 2016 May 5.
MicroRNAs (miRs) have been reported to be key regulators in numerous types of cancer. The aim of the present study was to investigate the role of miR-494 in ovarian cancer. Expression of miR-494 was analyzed in ovarian cancer tissues and cell lines by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). miR-494 mimic or negative control was transiently transfected into A2780 and SKOV3 cell lines. A cell counting kit-8 assay was performed to assess the effects of miR-494 on cell proliferation, and flow cytometry was used to evaluate the apoptotic rate. The target gene of miR-494 was detected by luciferase assay. Expression of fibroblast growth factor receptor 2 (FGFR2) was identified using RT-qPCR and western blotting. In the present study, decreased expression of miR-494 was observed in ovarian cancer samples and cell lines. Overexpression of miR-494 inhibited ovarian cancer cell proliferation by inducing apoptosis. Additional investigation indicated that FGFR2 was a direct target of miR-494. Taken together, the results of the present study suggested that miR-494 suppressed ovarian cancer cell proliferation by inducing apoptosis via targeting FGFR2.
据报道，微小RNA（miR）是多种癌症的关键调节因子。本研究的目的是探讨miR-494在卵巢癌中的作用。通过逆转录定量聚合酶链反应（RT-qPCR）分析miR-494在卵巢癌组织和细胞系中的表达。将miR-494模拟物或阴性对照瞬时转染到A2780和SKOV3细胞系中。进行细胞计数试剂盒-8检测以评估miR-494对细胞增殖的影响，并使用流式细胞术评估凋亡率。通过荧光素酶测定法检测miR-494的靶基因。使用RT-qPCR和蛋白质印迹法鉴定成纤维细胞生长因子受体2（FGFR2）的表达。在本研究中，观察到卵巢癌样本和细胞系中miR-494表达降低。miR-494的过表达通过诱导凋亡抑制卵巢癌细胞增殖。进一步研究表明，FGFR2是miR-494的直接靶标。综上所述，本研究结果表明，miR-494通过靶向FGFR2诱导凋亡来抑制卵巢癌细胞增殖。
