Izuchi Yukari, Takashima Tsuneo, Hatano Naoya
Technical Research Laboratory, Kaken Test Center.
Osaka Laboratory, Kaken Test Center.
Mass Spectrom (Tokyo). 2016;5(1):A0046. doi: 10.5702/massspectrometry.A0046. Epub 2016 Jun 6.
The demand for leather goods has grown globally in recent years. Industry revenue is forecast to reach $91.2 billion by 2018. There is an ongoing labelling problem in the leather items market, in that it is currently impossible to identify the species that a given piece of leather is derived from. To address this issue, we developed a rapid and simple method for the specific identification of leather derived from cattle, horses, pigs, sheep, goats, and deer by analysing peptides produced by the trypsin-digestion of proteins contained in leather goods using liquid chromatography/mass spectrometry. We determined species-specific amino acid sequences by liquid chromatography/tandem mass spectrometry analysis using the Mascot software program and demonstrated that collagen α-1(I), collagen α-2(I), and collagen α-1(III) from the dermal layer of the skin are particularly useful in species identification.
近年来，全球对皮革制品的需求不断增长。预计到2018年，行业收入将达到912亿美元。皮革制品市场目前存在一个标签问题，即目前无法识别某块皮革来自何种动物。为了解决这个问题，我们开发了一种快速简便的方法，通过使用液相色谱/质谱分析皮革制品中蛋白质经胰蛋白酶消化产生的肽段，来特异性鉴定牛、马、猪、羊、山羊和鹿的皮革。我们使用Mascot软件程序通过液相色谱/串联质谱分析确定了物种特异性氨基酸序列，并证明皮肤真皮层的胶原蛋白α-1(I)、胶原蛋白α-2(I)和胶原蛋白α-1(III)在物种鉴定中特别有用。
