Li Wen-Li, Zhao Xing-Chen, Zhao Zi-Wen, Huang Yan-Jun, Zhu Xuan-Zhi, Meng Ri-Zeng, Shi Ce, Yu Lu, Guo Na
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
b Jilin Enrty-exit Inspection and Quarantine Bureau , Changchun 130062 , China.
J Asian Nat Prod Res. 2016 Dec;18(12):1178-1185. doi: 10.1080/10286020.2016.1194829. Epub 2016 Jun 17.
Staphylococcus aureus (S. aureus) can attach to food, host tissues and the surfaces of medical implants and form a biofilm, which makes it difficult to eliminate. The purpose of this study was to evaluate the effect of honokiol on biofilm-grown S. aureus. In this report, honokiol showed effective antibacterial activity against S. aureus in biofilms. S. aureus isolates are capable of producing distinct types of biofilms mediated by polysaccharide intercellular adhesion (PIA) or extracellular DNA (eDNA). The biofilms' susceptibility to honokiol was evaluated using confocal laser scanning microscopy (CLSM) analysis. The transcript levels of the biofilm-related genes, the expression of PIA, and the amount of eDNA of biofilm-grown S. aureus exposed to honokiol were also investigated. The results of this study show that honokiol can detach existing biofilms, kill bacteria in biofilms, and simultaneously inhibit the transcript levels of sarA, cidA and icaA, eDNA release, and the expression of PIA.
金黄色葡萄球菌（S. aureus）可附着于食物、宿主组织及医用植入物表面并形成生物膜，这使得其难以被清除。本研究的目的是评估厚朴酚对生物膜生长的金黄色葡萄球菌的作用。在本报告中，厚朴酚对生物膜中的金黄色葡萄球菌显示出有效的抗菌活性。金黄色葡萄球菌分离株能够产生由多糖细胞间黏附（PIA）或细胞外DNA（eDNA）介导的不同类型的生物膜。使用共聚焦激光扫描显微镜（CLSM）分析评估生物膜对厚朴酚的敏感性。还研究了暴露于厚朴酚的生物膜生长的金黄色葡萄球菌的生物膜相关基因的转录水平、PIA的表达以及eDNA的量。本研究结果表明，厚朴酚可使现有生物膜脱落，杀死生物膜中的细菌，并同时抑制sarA、cidA和icaA的转录水平、eDNA释放以及PIA的表达。
