Meng Xin, Kang Mengna, Yu Zhiyun, Li Changyou, Chen Yang, Jin Taicheng, Wang Kai, Guo Haiyong
College of Life Science, Jilin Normal University, Siping, China.
RemeGen Co., Ltd., Yantai, China.
FEBS Open Bio. 2025 Apr;15(4):608-621. doi: 10.1002/2211-5463.13952. Epub 2024 Dec 15.
Methicillin-resistant Staphylococcus aureus (MRSA) poses a challenge for clinical treatment and combining antibiotics with other agents might be a promising strategy to overcome this challenge. This study explored the synergistic antibacterial activity of baicalin (traditional Chinese medicine extract) and the narrow-spectrum beta-lactam antibiotic oxacillin sodium, both of which are poorly active against MRSA in vitro. The combination of baicalin and oxacillin sodium showed a synergistic effect with a fractional inhibitory concentration index of 0.5. Mechanistically, the supplementation of baicalin increased the permeability of bacterial cell walls and cell membranes, enhancing oxacillin sodium entry and bactericidal action. The combination of baicalin and oxacillin sodium also significantly inhibited MRSA USA300 biofilm formation by further reducing polysaccharide intercellular adhesion production. Therefore, the combination of baicalin and oxacillin sodium offers a new therapeutic option for addressing clinical MRSA resistance. Further studies, including clinical trials, will be required to validate the observed in vitro results.
耐甲氧西林金黄色葡萄球菌（MRSA）对临床治疗构成挑战，将抗生素与其他药物联合使用可能是克服这一挑战的有效策略。本研究探讨了黄芩苷（一种中药提取物）与窄谱β-内酰胺类抗生素苯唑西林钠的协同抗菌活性，这两种药物在体外对MRSA的活性均较差。黄芩苷与苯唑西林钠联合使用表现出协同作用，部分抑菌浓度指数为0.5。从机制上讲，补充黄芩苷可增加细菌细胞壁和细胞膜的通透性，增强苯唑西林钠的进入及杀菌作用。黄芩苷与苯唑西林钠联合使用还通过进一步减少胞间多糖黏附的产生，显著抑制了MRSA USA300生物膜的形成。因此，黄芩苷与苯唑西林钠联合使用为解决临床MRSA耐药性提供了一种新的治疗选择。需要开展包括临床试验在内的进一步研究，以验证体外观察结果。
