Park C M, Hollenberg M J
Department of Anatomy, University of Toronto, Ontario, Canada.
Dev Biol. 1989 Jul;134(1):201-5. doi: 10.1016/0012-1606(89)90089-4.
In the present study, we have investigated the effect of basic fibroblast growth factor (bFGF) on retinal regeneration in the stage 22-24 chick embryo. The neural retina was surgically removed in ovo leaving the retinal pigment epithelium (RPE) intact and then slow-release, plastic implants containing bFGF were inserted into the eye. Light microscopic examination of eyes 7 days later revealed that bFGF induced retinal regeneration in a dose-dependent manner. The absence of the RPE in these eyes and the reversed polarity of the regenerated neural retina is consistent with the hypothesis that this process occurs by transdifferentiation of the RPE. This represents the first time that a known molecule has been shown to induce retinal regeneration in vivo.
在本研究中，我们研究了碱性成纤维细胞生长因子（bFGF）对22 - 24期鸡胚视网膜再生的影响。在鸡胚中通过手术切除神经视网膜，保留完整的视网膜色素上皮（RPE），然后将含有bFGF的缓释塑料植入物插入眼中。7天后对眼睛进行光学显微镜检查发现，bFGF以剂量依赖的方式诱导视网膜再生。这些眼中RPE的缺失以及再生神经视网膜的极性反转与RPE转分化导致该过程发生的假说一致。这是首次证明一种已知分子可在体内诱导视网膜再生。
