Alanio Alexandre, Sturny-Leclère Aude, Benabou Marion, Guigue Nicolas, Bretagne Stéphane
Parasitology-Mycology Laboratory, Lariboisière Saint-Louis Fernand Widal Hospitals, Assistance Publique-Hôpitaux de Paris (AP-HP), France; Université Paris-Diderot, Sorbonne Paris Cité, France; Institut Pasteur, Molecular Mycology Unit, CNRS URA3012, National Reference Center of Invasive Mycoses and Antifungals, France.
Institut Pasteur, Molecular Mycology Unit, CNRS URA3012, National Reference Center of Invasive Mycoses and Antifungals, France.
J Microbiol Methods. 2016 Aug;127:160-163. doi: 10.1016/j.mimet.2016.06.015. Epub 2016 Jun 14.
Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.
对10株无关的烟曲霉分离株进行检测时，DNA消化后的液滴数字PCR（ddPCR）得到的28S rDNA拷贝数为61至86拷贝/基因组，高于定量PCR。遗憾的是，在对侵袭性曲霉病血清患者进行检测时，DNA消化后的ddPCR并未提高我们PCR检测的灵敏度。
