Lee Hee-Seok, Park Eun-Jung, Han Songyi, Oh Gyeong-Yong, Kim Min-Hee, Kang Hui-Seung, Suh Jin-Hyang, Oh Jae-Ho, Lee Kwang-Soo, Hwang Myung-Sil, Moon Guiim, Hong Jin-Hwan, Hwang In-Gyun
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Food Chem Toxicol. 2016 Sep;95:121-7. doi: 10.1016/j.fct.2016.06.014. Epub 2016 Jun 16.
In this study, 27 chemicals found in household products, which became an issue in Korea were screened for the agonistoc and antagonistic effects against human estrogen receptor using official Organization for Economic Cooperation and Development (OECD) in vitro assays, STTA assay using ERα-HeLa-9903 cell line and BG1Luc ER TA assay. In the case of human ER agonist screening by two assays, all tested chemicals did not show agonist effect against ER. In ER antagonist test by BG1Luc ER TA assay, five surfactants α-dodecyl-ω-hydroxypoly(oxyethylene), alcohols C16-18 ethoxylated, nonylphenol, ethoxylated, 3,6,9,12,15,18,21-heptaoxatritriacontan-1-ol, and α-dodecyl-ω-hydroxypoly(oxy-1,2-ethanediyl)) were found to exhibit weak antagonistic activities. The agonist/antagonist effects against human estrogen receptor of various chemicals, used in Korea by OECD test guideline are reported in this study. These results indicated that two OECD in vitro assays will can be applied in Korea by screening of agonistic/antagonistic effects against human ER of various chemicals.
在本研究中，利用经济合作与发展组织（OECD）的官方体外试验、使用ERα-HeLa-9903细胞系的STTA试验以及BG1Luc ER TA试验，对在韩国引发问题的家用产品中发现的27种化学物质进行了针对人雌激素受体的激动和拮抗作用筛选。在通过两种试验进行人ER激动剂筛选的情况下，所有测试化学物质均未显示出对ER的激动作用。在通过BG1Luc ER TA试验进行的ER拮抗剂测试中，发现五种表面活性剂α-十二烷基-ω-羟基聚（氧乙烯）、C16 - 18乙氧基化醇、乙氧基化壬基酚、3,6,9,12,15,18,21 - 七氧杂三十三烷-1-醇和α-十二烷基-ω-羟基聚（氧-1,2-乙二基）表现出微弱的拮抗活性。本研究报告了按照经合组织测试指南在韩国使用的各种化学物质对人雌激素受体的激动/拮抗作用。这些结果表明，通过对各种化学物质对人ER的激动/拮抗作用进行筛选，两种经合组织体外试验可在韩国应用。
