Lee Hee-Seok, Park Eun-Jung, Han Songyi, Oh Gyeong-Yong, Kang Hui-Seung, Suh Jin-Hyang, Shin Min-Ki, Oh Hyun-Suk, Hwang Myung-Sil, Moon Guiim, Koh Young-Ho, Park Yooheon, Hong Jin-Hwan, Koo Yong Eui
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Korean Ministry of Food and Drug Safety, Chungcheongbuk-do 28159, South Korea.
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Korean Ministry of Food and Drug Safety, Chungcheongbuk-do 28159, South Korea.
Chemosphere. 2018 Jan;191:589-596. doi: 10.1016/j.chemosphere.2017.10.084. Epub 2017 Oct 14.
The aim of this study is to assess the androgen receptor (AR) agonistic/antagonistic effects on various chemicals, which are used in household products including cleaning agents and wetted tissues by in vitro OECD test guideline No. 458 (using AR-EcoScreen™ cell line) and the me-too test method (using 22Rv1cell line), which was adopted as OECD project No. 4.99. All chemicals were not determined as AR agonists. However α-dodecyl-ω-hydroxypoly (oxyethylene) and 3-iodo-2-propynyl butylcarbamate have shown a weak AR antagonistic effects with IC values of 2.18 ± 0.12 and 4.26 ± 0.17 μg/ml via binding affinity to AR in only 22Rv1/mouse mammary tumor virus using AR transcriptional activation assay, because of their different cytotoxicity on each applied cell line. This report firstly provides information about agonistic/antagonistic effects against human AR of various chemicals including surfactants and biocides by OECD in vitro stably transfected transcriptional activation assays. However, further in vivo and human model studies are needed to confirm their adverse effects.
本研究的目的是通过经合组织（OECD）第458号体外测试指南（使用AR-EcoScreen™细胞系）以及作为经合组织第4.99号项目采用的类似测试方法（使用22Rv1细胞系），评估雄激素受体（AR）对各种用于家用产品（包括清洁剂和湿纸巾）的化学品的激动/拮抗作用。所有化学品均未被确定为AR激动剂。然而，α-十二烷基-ω-羟基聚（氧乙烯）和3-碘-2-丙炔醇丁基氨酯通过仅在使用AR转录激活测定的22Rv1/小鼠乳腺肿瘤病毒中与AR的结合亲和力，显示出较弱的AR拮抗作用，IC值分别为2.18±0.12和4.26±0.17μg/ml，这是由于它们对每种应用细胞系的细胞毒性不同。本报告首次通过经合组织体外稳定转染转录激活测定，提供了包括表面活性剂和杀生物剂在内的各种化学品对人AR的激动/拮抗作用的信息。然而，需要进一步的体内和人体模型研究来确认它们的不利影响。
