IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany.
Curr Opin Biotechnol. 2016 Dec;42:169-177. doi: 10.1016/j.copbio.2016.05.005. Epub 2016 Jun 15.
Recent advances in biocatalysis have strongly boosted its recognition as a valuable addition to traditional chemical synthesis routes. As for any catalytic process, catalyst's costs and stabilities are of highest relevance for the economic application in chemical manufacturing. Employing biocatalysts as whole cells circumvents the need of cell lysis and enzyme purification and hence strongly cuts on cost. At the same time, residual cell wall components can shield the entrapped enzyme from potentially harmful surroundings and aid to enable applications far from natural enzymatic environments. Further advantages are the close proximity of reactants and catalysts as well as the inherent presence of expensive cofactors. Here, we review and comment on benefits and recent advances in whole cell biocatalysis.
生物催化技术的最新进展极大地提高了其作为传统化学合成路线的有益补充的认可度。对于任何催化过程而言，催化剂的成本和稳定性对于其在化学制造中的经济应用至关重要。使用完整细胞作为生物催化剂可以避免细胞裂解和酶纯化的需要，从而大大降低成本。同时，残留的细胞壁成分可以保护包裹的酶免受潜在有害环境的影响，并有助于实现远离自然酶环境的应用。进一步的优势是反应物和催化剂的紧密接近以及昂贵辅因子的固有存在。在这里，我们回顾和评论了完整细胞生物催化的优势和最新进展。
