Liu Haoran, Xu Kang, Sun ShuQi, Wan Yinbiao, Zhang Bojia, Song Yang, Guo Chuanzhuang, Sui Songsen, Wang Ruiming, Li Piwu, Wang Junqing, Xu Zhenshang, Wang Ting
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, People's Republic of China.
Shandong Freda Biotechnology Co. Ltd, Jinan, People's Republic of China.
Sci Rep. 2024 Dec 28;14(1):30771. doi: 10.1038/s41598-024-80561-5.
D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector. The recombinant E. coli strain pET22b(+) was successfully constructed and expressed, providing an efficient whole-cell catalyst for converting inexpensive D-fructose into D-allulose. Subsequently, we optimized the induction and incubation conditions step by step using the single-factor method and used Lactobacillus plantarum(LAB) 217-8 to enhance the purity of D-allulose in the system. Ultimately, the BL21/pET22b(+)-E. coli strain achieved a conversion rate of up to 33.91% under optimal conditions, converting D-fructose to D-allulose. After purification, the purity of D-allulose reached 64.73%. Efficient production of D-allulose is a significant achievement, paving the way for future probiotic applications in its conversion.
D-阿洛酮糖/D-阿洛糖是一种重要的稀有糖，在制药、食品和其他行业有广泛应用。在本研究中，我们以pET22b为载体，从球形节杆菌M30中克隆了D-阿洛酮糖3-表异构酶（DPEase）基因。成功构建并表达了重组大肠杆菌菌株pET22b(+)，为将廉价的D-果糖转化为D-阿洛酮糖提供了一种高效的全细胞催化剂。随后，我们采用单因素法逐步优化诱导和培养条件，并使用植物乳杆菌（LAB）217-8提高体系中D-阿洛酮糖的纯度。最终，BL21/pET22b(+)-大肠杆菌菌株在最佳条件下实现了高达33.91%的转化率，将D-果糖转化为D-阿洛酮糖。纯化后，D-阿洛酮糖的纯度达到64.73%。高效生产D-阿洛酮糖是一项重大成就，为其未来在益生菌转化中的应用铺平了道路。
