Engineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Department of Chemistry, Penn State University, University Park, PA 16802, USA; The Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Biomaterials. 2016 Sep;102:20-42. doi: 10.1016/j.biomaterials.2016.06.012. Epub 2016 Jun 7.
Droplet-based bioprinting (DBB) offers greater advantages due to its simplicity and agility with precise control on deposition of biologics including cells, growth factors, genes, drugs and biomaterials, and has been a prominent technology in the bioprinting community. Due to its immense versatility, DBB technology has been adopted by various application areas, including but not limited to, tissue engineering and regenerative medicine, transplantation and clinics, pharmaceutics and high-throughput screening, and cancer research. Despite the great benefits, the technology currently faces several challenges such as a narrow range of available bioink materials, bioprinting-induced cell damage at substantial levels, limited mechanical and structural integrity of bioprinted constructs, and restrictions on the size of constructs due to lack of vascularization and porosity. This paper presents a first-time review of DBB and comprehensively covers the existing DBB modalities including inkjet, electrohydrodynamic, acoustic, and micro-valve bioprinting. The recent notable studies are highlighted, the relevant bioink biomaterials and bioprinters are expounded, the application areas are presented, and the future prospects are provided to the reader.
液滴式生物打印（DBB）具有更高的优势，因为它具有简单性和灵活性，能够精确控制生物制剂的沉积，包括细胞、生长因子、基因、药物和生物材料，并且已经成为生物打印领域的一项突出技术。由于其巨大的多功能性，DBB 技术已被应用于多个应用领域，包括但不限于组织工程和再生医学、移植和临床、药物和高通量筛选以及癌症研究。尽管有很多好处，但该技术目前面临着几个挑战，例如可用的生物墨水材料范围有限、生物打印过程中细胞损伤程度较大、生物打印结构的机械和结构完整性有限，以及由于缺乏血管化和孔隙率而对构建体的大小造成限制。本文首次对 DBB 进行了综述，全面涵盖了现有的 DBB 方式，包括喷墨、电动力学、声和微阀生物打印。突出了最近的显著研究，阐述了相关的生物墨水生物材料和生物打印机，展示了应用领域，并为读者提供了未来的展望。
