Ajji Parminder Kaur, Walder Ken, Puri Munish
Bioprocessing Laboratory, Centre for Chemistry and Biotechnology, School of Life and Environment Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, Geelong, 3220, Victoria, Australia.
Metabolic Research Unit, School of Medicine, Deakin University, Waurn Ponds, 75 Pigdons Road, Locked Bag 20000, Geelong, VIC, 3220, Australia.
Plant Foods Hum Nutr. 2016 Sep;71(3):265-71. doi: 10.1007/s11130-016-0555-4.
Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg(2+) (10-50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
核糖体失活蛋白（RIPs）因其对肿瘤细胞和病毒感染细胞的独特作用，在生物医学研究中受到了广泛关注。我们从苦瓜中提取了一种I型RIP——苦瓜素。在本研究中，我们使用纯化的苦瓜素对其脱氧核糖核酸酶（DNase）活性、抗氧化能力和抗菌活性进行了详细研究。苦瓜素对质粒DNA的类DNase活性依赖于pH值、孵育时间和温度。此外，镁离子（Mg²⁺，10 - 50 mM）的存在会影响DNA切割活性。苦瓜素还表现出还原能力，并能以剂量依赖的方式清除自由基。此外，该蛋白对金黄色葡萄球菌、肠炎沙门氏菌、表皮葡萄球菌和大肠杆菌具有抗菌活性，这表明苦瓜素作为一种营养保健品具有潜在的应用价值。
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