Ajji Parminder K, Walder Ken, Puri Munish
Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC, Australia.
Centre for Molecular and Medical Research, School of Medicine, Deakin University, Geelong, VIC, Australia.
Front Pharmacol. 2020 Oct 28;11:574496. doi: 10.3389/fphar.2020.574496. eCollection 2020.
Flavonoids such as naringenin, quercetin, and naringin are known to exhibit anticancer properties. In this study, we examined the effects of these flavonoids on cell viability and apoptotic pathways of cancer cells, either singly or in combination with the type 1 ribosome inactivating protein, Balsamin. Treatment with flavonoids (naringenin, quercetin, and naringin) plus Balsamin for 48 h reduced HepG2 and MCF-7 cell viability, increased the activation of caspase-3 and -8, and induced apoptosis through up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic genes. Out of the three flavonoids tested, the Balsamin-Naringenin and Balsamin-Quercetin combinations appeared to be most effective compared to the Balsamin-Naringin combination. Balsamin combined with flavonoids also activated endoplasmic reticulum (ER)-stress-mediated apoptosis in breast cancer (MCF-7) cells, which was not activated by Balsamin treatment alone. These experimental results showed that Balsamin combined with flavonoids can reduce HepG2 and MCF-7 cells viability and induce apoptosis, which could be considered as a promising therapeutic approach to sensitize cells to Balsamin treatment, thereby improving its efficacy in breast or liver cancer therapy.
已知柚皮素、槲皮素和柚皮苷等类黄酮具有抗癌特性。在本研究中，我们研究了这些类黄酮单独或与1型核糖体失活蛋白苦瓜素联合使用时，对癌细胞的细胞活力和凋亡途径的影响。用类黄酮（柚皮素、槲皮素和柚皮苷）加苦瓜素处理48小时可降低HepG2和MCF-7细胞的活力，增加caspase-3和-8的激活，并通过上调促凋亡基因和下调抗凋亡基因诱导细胞凋亡。在测试的三种类黄酮中，与苦瓜素-柚皮苷组合相比，苦瓜素-柚皮素和苦瓜素-槲皮素组合似乎最有效。苦瓜素与类黄酮联合使用还可激活乳腺癌（MCF-7）细胞中内质网（ER）应激介导的凋亡，而单独使用苦瓜素处理则不会激活这种凋亡。这些实验结果表明，苦瓜素与类黄酮联合使用可降低HepG2和MCF-7细胞的活力并诱导细胞凋亡，这可被视为一种有前景的治疗方法，可使细胞对苦瓜素治疗敏感，从而提高其在乳腺癌或肝癌治疗中的疗效。
