Langemann H, Torhorst J, Kabiersch A, Krenger W, Honegger C G
Research Department, Cantonal Hospital, University of Basel, Switzerland.
Int J Cancer. 1989 Jun 15;43(6):1169-73. doi: 10.1002/ijc.2910430634.
Ascorbic acid, cysteine, glutathione and uric acid were determined by reverse-phase high-pressure liquid chromatography (HPLC) in 46 breast tissue samples [neoplastic (C) and non-neoplastic (N) from the same patient]. Cholesterol, alpha-tocopherol and gamma-tocopherol were quantified in 64 similar samples by extraction into heptane followed by direct-phase HPLC. DNA was measured in all samples and the percentages of epithelium, fat and connective tissue were estimated in sections adjacent to the sample. Results confirm previous findings that ascorbic acid and glutathione, expressed as mumol/g DNA, were greatly increased in the epithelium of neoplastic tissue. Similar increases in cysteine could be accounted for by the presence of inflammatory cells. Although values of alpha- and gamma-tocopherol correlated with the percentage of fat in both types of tissue, these compounds were also present in the epithelium. Because of the varying amounts of fat in the samples, no significant difference could be found between N and C values. Cholesterol correlated with fat in N and epithelium in C. Consideration of 10 cases with equal amounts of fat in C and N tissue suggests that cholesterol is reduced in C in the epithelial cells.
采用反相高效液相色谱法（HPLC）对46份乳腺组织样本[来自同一患者的肿瘤性（C）和非肿瘤性（N）样本]中的抗坏血酸、半胱氨酸、谷胱甘肽和尿酸进行了测定。通过用庚烷萃取，随后进行正相HPLC，对64份类似样本中的胆固醇、α-生育酚和γ-生育酚进行了定量。对所有样本进行了DNA测定，并在与样本相邻的切片中估计了上皮组织、脂肪组织和结缔组织的百分比。结果证实了先前的研究结果，即肿瘤组织上皮中以μmol/g DNA表示的抗坏血酸和谷胱甘肽大幅增加。半胱氨酸的类似增加可能是由于炎症细胞的存在。尽管α-生育酚和γ-生育酚的值与两种组织中的脂肪百分比相关，但这些化合物也存在于上皮组织中。由于样本中脂肪含量不同，N和C值之间未发现显著差异。胆固醇与N中的脂肪和C中的上皮组织相关。对10例C和N组织中脂肪含量相等的病例进行分析表明，C的上皮细胞中胆固醇减少。
