Honegger C G, Torhorst J, Langemann H, Kabiersch A, Krenger W
Research Department, Cantonal Hospital, University of Basle, Switzerland.
Int J Cancer. 1988 May 15;41(5):690-4. doi: 10.1002/ijc.2910410509.
The water-soluble scavengers ascorbic acid (Asc), cysteine (Cys), glutathione (GSH) and uric acid (UA) as well as DNA content were determined in 40 breast tissue samples (neoplastic and non-neoplastic tissues from 20 patients). To allow proper homogenization to take place, a fixed number of sections was cut from a tissue cylinder of known diameter. Adjacent sections were stained with hematoxylin-eosin and the fractions of epithelium, fat and connective tissue were estimated as a percentage of the section area. Protein-free extracts were injected into a reverse-phase high-pressure liquid chromatography system and scavengers quantified with 2 electrochemical detectors (gold and glassy carbon). DNA and all scavengers, except UA, were greatly increased in cancer tissues in nearly all cases. Amounts of Asc and GSH in neoplastic tissue correlated closely with DNA values and percentage of epithelium, those of Cys not so closely and those of UA not at all. We assume that Asc and GSH were located mainly in the epithelium, UA mainly in the extracellular space and Cys in both spaces. When values were expressed as mumol/g DNA, a parameter related to content per cell, values were higher in neoplastic than in non-neoplastic tissue for Asc (18/20 cases), GSH (17/20) and Cys (14/20) and lower in neoplastic tissue for UA (19/20). It is known that increased GSH protects cells against certain drugs in tissue cultures. For in vivo treatment the presence of increased Asc (and to a lesser extent Cys) in addition to GSH could be of importance.
在40份乳腺组织样本（来自20名患者的肿瘤组织和非肿瘤组织）中测定了水溶性清除剂抗坏血酸（Asc）、半胱氨酸（Cys）、谷胱甘肽（GSH）和尿酸（UA）以及DNA含量。为了实现适当的匀浆，从已知直径的组织圆柱体上切下固定数量的切片。相邻切片用苏木精-伊红染色，并估计上皮、脂肪和结缔组织的比例占切片面积的百分比。将无蛋白提取物注入反相高压液相色谱系统，并用2个电化学检测器（金和玻碳）对清除剂进行定量。在几乎所有情况下，癌症组织中的DNA以及除UA外的所有清除剂均大幅增加。肿瘤组织中Asc和GSH的含量与DNA值和上皮细胞百分比密切相关，Cys的相关性不那么密切，UA则完全不相关。我们假设Asc和GSH主要位于上皮细胞中，UA主要位于细胞外空间，Cys在这两个空间中均有分布。当以μmol/g DNA表示数值时（这是一个与每个细胞含量相关的参数），肿瘤组织中Asc（18/20例）、GSH（17/20）和Cys（14/20）的值高于非肿瘤组织，而UA在肿瘤组织中的值较低（19/20）。已知在组织培养中增加GSH可保护细胞免受某些药物的影响。对于体内治疗，除了GSH外，增加Asc（以及在较小程度上增加Cys）的存在可能很重要。
