Xie Qiong, Pan Mingfang, Huang Renhui, Tian Ximei, Tao Xueying, Shah Nagendra P, Wei Hua, Wan Cuixiang
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
College of Life Science, Nanchang University, Nanchang 330031, P. R. China.
J Dairy Sci. 2016 Sep;99(9):6913-6921. doi: 10.3168/jds.2016-11141. Epub 2016 Jun 16.
The small intestinal (SI) microbiota has an essential role in the maintenance of human health. However, data about the indigenous bacteria in SI as affected by probiotics are limited. In our study, the short-term and long-term effects of a probiotic candidate, Lactobacillus plantarum ZDY2013, on the SI microbiota of C57BL/6J mice were investigated by the Illumina HiSeq (Novogene Bioinformatics Technology Co., Ltd., Tianjin, China) platform targeting the V4 region of the 16S rDNA. A total of 858,011 sequences in 15 samples were read. The α diversity analysis revealed that oral administration with L. plantarum ZDY2013 for 3 wk led to a significant increase in the richness and diversity of the SI bacterial community. Principal coordinate analysis and unweighted pair-group method with arithmetic means analysis showed a clear alteration in the SI microbiota composition after 3 wk of L. plantarum ZDY2013 treatment, although these changes were not found 6 wk after ceasing L. plantarum ZDY2013 administration. Species annotation showed that the dominant phyla in SI microbiota were Firmicutes, Bacteroidetes, Proteobacteria, and Verrucomicrobia. Interestingly, operational taxonomic unit cluster analysis showed that administration with L. plantarum ZDY2013 for 3 wk significantly increased the abundance of Proteobacteria, but decreased that of Bacteroidetes. Linear discriminant analysis coupled with effect size identified 18 bacterial taxa (e.g., Ruminococcus spp. and Clostridium spp.) that overgrew in the SI microbiota of the mice administered with L. plantarum ZDY2013 for 3 wk, and most of them belonged to the phyla Bacteroidetes and Proteobacteria. However, only one bacterial taxon (e.g., Nocardioides spp.) was over-represented in the SI microbiota of mice 6 wk after L. plantarum ZDY2013 administration. Overall, this study shows that oral administration with probiotic results in an important but transient alteration in the microbiota of SI.
小肠（SI）微生物群在维持人类健康方面起着至关重要的作用。然而，关于益生菌对SI中本土细菌影响的数据有限。在我们的研究中，通过针对16S rDNA V4区域的Illumina HiSeq（中国天津诺禾致源生物信息科技有限公司）平台，研究了候选益生菌植物乳杆菌ZDY2013对C57BL/6J小鼠SI微生物群的短期和长期影响。共读取了15个样本中的858,011条序列。α多样性分析表明，口服植物乳杆菌ZDY2013 3周导致SI细菌群落的丰富度和多样性显著增加。主坐标分析和算术平均非加权配对组方法分析表明，植物乳杆菌ZDY2013处理3周后，SI微生物群组成发生了明显变化，尽管在停止施用植物乳杆菌ZDY2013 6周后未发现这些变化。物种注释表明，SI微生物群中的优势菌门为厚壁菌门、拟杆菌门、变形菌门和疣微菌门。有趣的是，操作分类单元聚类分析表明，施用植物乳杆菌ZDY2013 3周显著增加了变形菌门的丰度，但降低了拟杆菌门的丰度。线性判别分析结合效应大小确定了18个细菌分类单元（如瘤胃球菌属和梭菌属），这些分类单元在施用植物乳杆菌ZDY2013 3周的小鼠SI微生物群中过度生长，其中大多数属于拟杆菌门和变形菌门。然而，在施用植物乳杆菌ZDY2013 6周后的小鼠SI微生物群中，只有一个细菌分类单元（如诺卡氏菌属）的含量过高。总体而言，这项研究表明，口服益生菌会导致SI微生物群发生重要但短暂的变化。
