Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Shandong Linyi Tobacco Co., Ltd., Linyi 276003, China.
Biosensors (Basel). 2022 May 14;12(5):338. doi: 10.3390/bios12050338.
As a common herbicide in farmland, there has been wide concern over quinclorac residue because of its potential risks to the environment and human health. For the detection and monitoring of quinclorac residue in the environment, enzyme-linked immunoassay (ELISA) and time-resolved fluoroimmunoassay (TRFIA) were established. The half-maximal inhibition concentrations (IC50) of ELISA and TRFIA were 0.169 mg/L and 0.087 mg/L with a linear range (IC20−IC80) of 0.020−1.389 mg/L and 0.004−1.861 mg/L, respectively. Compared with ELISA, the limit of detection (LOD, IC20) and IC50 of TRFIA improved approximately 5-fold and 2-fold. The cross-reaction rates for the quinclorac analogs were less than 2%. The average recoveries of quinclorac in river water, paddy water, paddy soil, and brown rice samples were 77.3−106.1%, with RSDs of 1.7−12.5%. More importantly, the results of the two methods were consistent with that of the referenced method of UPLC-MS/MS (R2 > 0.98). ELISA and TRFIA both showed good detection performance and could meet the requirements of the quantitative determination of quinclorac. Therefore, the proposed ELISA and TRFIA could be applied to the rapid and sensitive detection and monitoring of quinclorac residue in the environment.
作为农田中常用的除草剂，由于其对环境和人类健康的潜在风险，草除灵残留问题受到了广泛关注。为了检测和监测环境中的草除灵残留，建立了酶联免疫吸附测定法（ELISA）和时间分辨荧光免疫分析法（TRFIA）。ELISA 和 TRFIA 的半抑制浓度（IC50）分别为 0.169mg/L 和 0.087mg/L，线性范围（IC20-IC80）分别为 0.020-1.389mg/L 和 0.004-1.861mg/L。与 ELISA 相比，TRFIA 的检测限（IC20）和 IC50 分别提高了约 5 倍和 2 倍。草除灵类似物的交叉反应率均小于 2%。河水、稻田水、稻田土和糙米样品中草除灵的平均回收率为 77.3-106.1%，相对标准偏差（RSD）为 1.7-12.5%。更重要的是，两种方法的结果与 UPLC-MS/MS 参考方法一致（R2>0.98）。ELISA 和 TRFIA 均表现出良好的检测性能，可满足草除灵定量测定的要求。因此，所提出的 ELISA 和 TRFIA 可用于环境中草除灵残留的快速灵敏检测和监测。
