Wang Y, Luo X, Zhang G H, Li S L
Department of Radiation Medicine, Chongqing Medical University, Chongqing, China.
Department of Preventive Medicine, North Sichuan Medical College, Nanchong, Sichuan, China.
Genet Mol Res. 2016 May 23;15(2):gmr7796. doi: 10.4238/gmr.15027796.
Proton nuclear magnetic resonance ([(1)H]-NMR) spectroscopy has been used to investigate metabolites in serum and several types of tissue. We used NMR spectroscopy to explore the differential metabolic profiles in serum from nasopharyngeal carcinoma (NPC) patients. Moreover, metabolites with potential as biomarkers for identifying NPC patients were primarily identified. Serum samples were collected from 40 enrolled participants comprising 20 healthy subjects and 20 NPC patients. Samples were analyzed using a 600-MHz NMR spectrometer. The [(1)H]-NMR spectra were further analyzed with partial least squares-discriminant analysis for screening differential metabolites. NMR spectroscopy identified a total of eight metabolites that were present at different levels when the sera of NPC patients were compared with those of healthy individuals. Methionine, taurine (P < 0.05), and choline-like metabolites (P < 0.05) were mostly elevated in the sera of NPC patients. In contrast, the levels of lipids (P < 0.01), isoleucine (P < 0.05), unsaturated lipids (P < 0.01), trimethylamine oxidase (P < 0.05), and carbohydrates (P < 0.05) were lower in the sera of the NPC patients than in the healthy controls. We explored the differential metabolic profiles in sera from NPC patients. [(1)H]-NMR spectroscopy can be used to identify specific metabolites, and is capable of distinguishing between NPC patients and healthy individuals.
质子核磁共振（[(1)H]-NMR）光谱已被用于研究血清和几种组织类型中的代谢物。我们使用NMR光谱来探索鼻咽癌（NPC）患者血清中的差异代谢谱。此外，初步鉴定了具有作为识别NPC患者生物标志物潜力的代谢物。从40名登记参与者中收集血清样本，其中包括20名健康受试者和20名NPC患者。使用600-MHz NMR光谱仪对样本进行分析。[(1)H]-NMR光谱通过偏最小二乘判别分析进一步分析，以筛选差异代谢物。NMR光谱共鉴定出8种代谢物，当将NPC患者的血清与健康个体的血清进行比较时，这些代谢物的含量存在差异。蛋氨酸、牛磺酸（P < 0.05）和类胆碱代谢物（P < 0.05）在NPC患者血清中大多升高。相比之下，NPC患者血清中脂质（P < 0.01）、异亮氨酸（P < 0.05）、不饱和脂质（P < 0.01）、三甲胺氧化酶（P < 0.05）和碳水化合物（P < 0.05）的水平低于健康对照组。我们探索了NPC患者血清中的差异代谢谱。[(1)H]-NMR光谱可用于识别特定代谢物，并且能够区分NPC患者和健康个体。
