Wang Zhong-Shan, Feng Zhi-Hong, Wu Guo-Feng, Bai Shi-Zhu, Dong Yan, Chen Fa-Ming, Zhao Yi-Min
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Sci Rep. 2016 Jun 21;6:28126. doi: 10.1038/srep28126.
Periodontal regeneration involves the restoration of at least three unique tissues: cementum, periodontal ligament tissue (PDL) and alveolar bone tissue. Here, we first isolated human PDL stem cells (PDLSCs) and jaw bone mesenchymal stem cells (JBMSCs). These cells were then induced to form cell sheets using an ascorbic acid-rich approach, and the cell sheet properties, including morphology, thickness and gene expression profile, were compared. Platelet-rich fibrin (PRF) derived from human venous blood was then fabricated into bioabsorbable fibrin scaffolds containing various growth factors. Finally, the in vivo potential of a cell-material construct based on PDLSC sheets, PRF scaffolds and JBMSC sheets to form periodontal tissue was assessed in a nude mouse model. In this model, PDLSC sheet/PRF/JBMSC sheet composites were placed in a simulated periodontal space comprising human treated dentin matrix (TDM) and hydroxyapatite (HA)/tricalcium phosphate (TCP) frameworks. Eight weeks after implantation, the PDLSC sheets tended to develop into PDL-like tissues, while the JBMSC sheets tended to produce predominantly bone-like tissues. In addition, the PDLSC sheet/PRF/JBMSC sheet composites generated periodontal tissue-like structures containing PDL- and bone-like tissues. Further improvements in this cell transplantation design may have the potential to provide an effective approach for future periodontal tissue regeneration.
牙骨质、牙周韧带组织（PDL）和牙槽骨组织。在此，我们首先分离出人牙周膜干细胞（PDLSCs）和颌骨间充质干细胞（JBMSCs）。然后使用富含抗坏血酸的方法诱导这些细胞形成细胞片，并比较细胞片的特性，包括形态、厚度和基因表达谱。接着将源自人静脉血的富血小板纤维蛋白（PRF）制成含有各种生长因子的可生物吸收纤维蛋白支架。最后，在裸鼠模型中评估基于PDLSC片、PRF支架和JBMSC片的细胞 - 材料构建体形成牙周组织的体内潜力。在该模型中，将PDLSC片/PRF/JBMSC片复合材料置于包含人处理过的牙本质基质（TDM）和羟基磷灰石（HA）/磷酸三钙（TCP）框架的模拟牙周间隙中。植入八周后，PDLSC片倾向于发育成类似PDL的组织，而JBMSC片则主要倾向于产生类似骨的组织。此外，PDLSC片/PRF/JBMSC片复合材料生成了包含类似PDL和骨组织的牙周组织样结构。这种细胞移植设计的进一步改进可能为未来的牙周组织再生提供一种有效的方法。
