Frade Taíssa Iolanda Checón, Dos Reis Diego Carlos, Cassali Geovanni Dantas, Bakhle Yeshwant S, de Francischi Janetti Nogueira
Dentistry Faculty at Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
General Pathology Department of Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Inflammopharmacology. 2016 Aug;24(4):145-53. doi: 10.1007/s10787-016-0269-0. Epub 2016 Jun 20.
In the current study, carrageenan (CG; 100-1000 μg/site) was injected intraorally in the cheeks of Holtzman or Wistar rats to evaluate the consequences of administration of a non-immunogenic stimulus in the orofacial region. Subsequent inflammation was measured as oedema (increased thickness of the cheek wall using digital calipers), relative to the other cheek injected with saline. Oedema formation and tissue collection for histopathological studies were assessed at 0.5, 1, 2, 3, 4, 6, 24, 48, 72, 96, 120 and 144 h after injection. In parallel, other groups of rats were injected with CG in the hind paw, to provide a reference response. The inhibitor of prostaglandin biosynthesis, indomethacin, and antagonists of histamine, serotonin and NK1 receptors were injected s.c., 0.5 h before CG. CG induced a dose-related oedema more rapidly from 0 to 2 h which lasted for at least 72 h, showing a biphasic profile (peak at 2 and 24 h), compared with the monophasic oedema induced in rat paws (maximal duration of 24 h). Histopathological analysis of the CG-injected cheek revealed oedema formation with little leukocyte recruitment at 1-3 h, mast cell degranulation at 6 h, and a mixed polymorphonuclear and mononuclear cell infiltrate by 24 h. Histamine and serotonin antagonists and indomethacin, but not the NK1 antagonist, decreased cheek oedema in the first 4 h following carrageenan. Taken together, our data indicated important differences in the pattern of inflammation between the oral cavity and the paw which will determine the therapeutic approach to the treatment of inflammatory conditions in the oral cavity.
在本研究中，将角叉菜胶（CG；100 - 1000μg/部位）经口注射到霍尔茨曼大鼠或Wistar大鼠的脸颊，以评估在口面部区域给予非免疫原性刺激的后果。相对于注射生理盐水的另一侧脸颊，随后将炎症测量为水肿（使用数字卡尺测量脸颊壁厚度增加）。在注射后0.5、1、2、3、4、6、24、48、72、96、120和144小时评估水肿形成情况并收集组织用于组织病理学研究。同时，将其他组大鼠的后爪注射CG，以提供参考反应。在注射CG前0.5小时皮下注射前列腺素生物合成抑制剂吲哚美辛以及组胺、5-羟色胺和NK1受体拮抗剂。与在大鼠爪子中诱导的单相水肿（最大持续时间为24小时）相比，CG在0至2小时内更快地诱导出剂量相关的水肿，该水肿持续至少72小时，呈现双相特征（在2小时和24小时达到峰值）。对注射CG的脸颊进行组织病理学分析显示，在1 - 3小时出现水肿形成且白细胞募集较少，在6小时出现肥大细胞脱颗粒，到24小时出现多形核细胞和单核细胞混合浸润。组胺和5-羟色胺拮抗剂以及吲哚美辛，但不是NK1拮抗剂，在注射角叉菜胶后的前4小时减少了脸颊水肿。综上所述，我们的数据表明口腔和爪子的炎症模式存在重要差异，这将决定口腔炎症性疾病的治疗方法。
