海胆精子结合素的卵表面糖蛋白受体。
Egg surface glycoprotein receptor for sea urchin sperm bindin.
作者信息
Glabe C G, Vacquier V D
出版信息
Proc Natl Acad Sci U S A. 1978 Feb;75(2):881-5. doi: 10.1073/pnas.75.2.881.
Abstract
Bindin is an insoluble protein coating the sperm acrosome process and mediating the adhesion of sperm to sea urchin eggs. Milligrams of bindin have been isolated. Here we report the identification, isolation, and partial characterization of a high molecular weight, trypsin-sensitive glycoprotein fraction from the sea urchin egg surface having species-specific affinity for bindin. This glycoprotein may be the egg surface receptor for bindin. The bindin receptor was released from 125-I-labeled eggs by parthenogenetic activation of eggs with ionophore A23187 in the presence of soybean trypsin inhibitor. The receptor has an isoelectric point of 4.02 and a molecular weight in sea water greater than or equal to 5 X 10(6), suggesting that it is an aggregate. It contains 34% neutral sugars, which are galactose and mannose.
摘要
结合蛋白是一种不溶性蛋白质，覆盖在精子顶体突起上，介导精子与海胆卵的黏附。已经分离出了毫克级的结合蛋白。在此，我们报告了从海胆卵表面鉴定、分离和部分表征一种高分子量、对胰蛋白酶敏感的糖蛋白组分，该组分对结合蛋白具有物种特异性亲和力。这种糖蛋白可能是结合蛋白的卵表面受体。在大豆胰蛋白酶抑制剂存在的情况下，用离子载体A23187对卵进行孤雌激活，可从125-I标记的卵中释放出结合蛋白受体。该受体的等电点为4.02，在海水中的分子量大于或等于5×10(6)，表明它是一种聚集体。它含有34%的中性糖，即半乳糖和甘露糖。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Analysis of neutral sugars by gas-liquid chromatography of alditol acetates: application to thyrotropic hormone and other glycoproteins.通过糖醇乙酸酯的气液色谱法分析中性糖：在促甲状腺激素和其他糖蛋白中的应用
Anal Biochem. 1967 Aug;20(2):258-74. doi: 10.1016/0003-2697(67)90031-0.
3
Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy.受精时从海胆卵中释放出的蛋白酶会改变卵黄膜，并有助于防止多精受精。
Exp Cell Res. 1973 Jul;80(1):111-9. doi: 10.1016/0014-4827(73)90281-4.
4
Physiological studies on the sperm surface component responsible for sperm-egg bonding in sea urchin fertilization. I. Effect of sperm-binding protein on the fertilizing capacity of sperm.海胆受精过程中负责精卵结合的精子表面成分的生理学研究。I. 精子结合蛋白对精子受精能力的影响。
Exp Cell Res. 1973 Aug;80(2):439-41. doi: 10.1016/0014-4827(73)90317-0.
5
Heat sensitivity of the cortical granule protease from hamster eggs.仓鼠卵皮质颗粒蛋白酶的热敏感性
J Reprod Fertil. 1974 Jul;39(1):153-5. doi: 10.1530/jrf.0.0390153.
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Methods for quantitating sea urchin sperm-egg binding.海胆精卵结合定量方法。
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7
Sea urchin eggs release protease activity at fertilization.海胆卵在受精时释放蛋白酶活性。
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