Generoso Wesley Cardoso, Gottardi Manuela, Oreb Mislav, Boles Eckhard
Institute of Molecular Biosciences, Goethe University Frankfurt, 60438 Frankfurt am Main, Germany.
J Microbiol Methods. 2016 Aug;127:203-205. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
CRISPR-Cas has become a powerful technique for genetic engineering of yeast. Here, we present an improved version by using only one single plasmid expressing Cas9 and one or two guide-RNAs. A high gene deletion efficiency was achieved even with simultaneous recombination cloning of the plasmid and deletion in industrial strains.
CRISPR-Cas已成为用于酵母基因工程的一项强大技术。在此，我们展示了一个改进版本，该版本仅使用一个表达Cas9的单一质粒和一个或两个引导RNA。即使在工业菌株中同时进行质粒的重组克隆和基因缺失，也能实现较高的基因缺失效率。
