阿尔茨海默病患者泪液化学屏障成分的变化揭示了潜在的泪液诊断生物标志物。
Changes in the Chemical Barrier Composition of Tears in Alzheimer's Disease Reveal Potential Tear Diagnostic Biomarkers.
作者信息
Kalló Gergő, Emri Miklós, Varga Zsófia, Ujhelyi Bernadett, Tőzsér József, Csutak Adrienne, Csősz Éva
机构信息
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem ter 1., 4032 Debrecen, Hungary.
Department of Nuclear Medicine, Faculty of Medicine, University of Debrecen, Egyetem ter 1., 4032 Debrecen, Hungary.
出版信息
PLoS One. 2016 Jun 21;11(6):e0158000. doi: 10.1371/journal.pone.0158000. eCollection 2016.
Alzheimer's disease (AD) is one of the most common neurodegenerative diseases, with increasing prevalence affecting millions of people worldwide. Currently, only autopsy is able to confirm the diagnosis with a 100% certainty, therefore, biomarkers from body fluids obtained by non-invasive means provide an attractive alternative for the diagnosis of Alzheimer`s disease. Global changes of the protein profile were examined by quantitative proteomics; firstly, electrophoresis and LC-MS/MS were used, thereafter, SRM-based targeted proteomics method was developed and applied to examine quantitative changes of tear proteins. Alterations in the tear flow rate, total tear protein concentration and composition of the chemical barrier specific to AD were demonstrated, and the combination of lipocalin-1, dermcidin, lysozyme-C and lacritin was shown to be a potential biomarker, with an 81% sensitivity and 77% specificity.
阿尔茨海默病（AD）是最常见的神经退行性疾病之一，其患病率不断上升，影响着全球数百万人。目前，只有尸检能够100%确诊，因此，通过非侵入性手段从体液中获取的生物标志物为阿尔茨海默病的诊断提供了一种有吸引力的替代方法。通过定量蛋白质组学研究了蛋白质谱的整体变化；首先，使用了电泳和液相色谱-串联质谱，随后，开发了基于选择反应监测的靶向蛋白质组学方法并应用于检测泪液蛋白质的定量变化。结果表明，泪液流速、总泪液蛋白浓度以及AD特有的化学屏障组成发生了改变，脂质运载蛋白-1、皮肤杀菌素、溶菌酶-C和乳铁蛋白的组合被证明是一种潜在的生物标志物，灵敏度为81%，特异性为77%。
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