Zhang Yilin, Hou Weiwei, Liu Yang, Long Hui, Zhang Ling, Zhu Zhuoli, Yu Haiyang
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China; Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, PR China.
Arch Oral Biol. 2016 Oct;70:47-54. doi: 10.1016/j.archoralbio.2016.06.009. Epub 2016 Jun 8.
Previous studies have demonstrated that low-magnitude, high-frequency (LMHF) mechanical vibrations (LM<1g; HF<20-90Hz) can benefit bone formation. However, the underlying mechanism is unclear. In this study, MC3T3-E1 cells were exposed to LMHF vibrations of different magnitudes to assess the effect of vibrations on β-catenin expression, which regulates osteogenic differentiation. LMHF vibrations (0.14-0.49g)increased cell viability, alkaline phosphatase activity and calcification in a dose (magnitude)-dependent manner. The level of β-catenin expression increased 1.6-fold using 0.49g LMHF vibrations compared to the control. Similarly, the use of LMHF vibrations increased Runx2 protein expression with the greatest effect at 0.32g exposure. The levels of BMP2, Osterix and Cyclin D1 protein expression were also elevated and were expressed highest in cells exposed to 0.49g. mRNA expression levels of these proteins were similarly affected by exposure to LMHF vibrations. These results suggest that LMHF vibrations promote cell viability and osteogenic differentiation of osteoblasts by up-regulating related proteins and genes. The activation of β-catenin plays an important role in the effect of microvibration exposure in osteoblasts.
先前的研究表明，低强度、高频（LMHF）机械振动（LM<1g；HF<20 - 90Hz）可促进骨形成。然而，其潜在机制尚不清楚。在本研究中，将MC3T3 - E1细胞暴露于不同强度的LMHF振动下，以评估振动对调控成骨分化的β - 连环蛋白表达的影响。LMHF振动（0.14 - 0.49g）以剂量（强度）依赖性方式提高了细胞活力、碱性磷酸酶活性和钙化程度。与对照组相比，使用0.49g的LMHF振动使β - 连环蛋白表达水平增加了1.6倍。同样，使用LMHF振动可增加Runx2蛋白表达，在0.32g暴露时效果最为显著。BMP2、Osterix和细胞周期蛋白D1的蛋白表达水平也有所升高，且在暴露于0.49g的细胞中表达最高。这些蛋白质的mRNA表达水平同样受到LMHF振动暴露的影响。这些结果表明，LMHF振动通过上调相关蛋白质和基因来促进成骨细胞的细胞活力和成骨分化。β - 连环蛋白的激活在成骨细胞微振动暴露的效应中起重要作用。
