Padmanabhan Aiswaria, Hause Ben M
Kansas State Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, Kansas State University, 1800 Denison Avenue, Manhattan, Kansas, 66506, USA.
Arch Virol. 2016 Sep;161(9):2575-9. doi: 10.1007/s00705-016-2937-1. Epub 2016 Jun 21.
Metagenomic sequencing of three nasal swabs collected from 10- to 21-day-old pigs exhibiting unexplained acute respiratory disease from two different commercial production facilities in Oklahoma identified a novel genotype of porcine astrovirus 4 (PAstV-4). The genomes had only ~75 % nucleotide sequence identity to previously characterized PAstV-4 isolates, while the capsid-encoding ORF2 had only ~53 % amino acid sequence identity to reference strains. A TaqMan assay targeting the novel ORF2 gene found 21 % and 19 % incidence in nasal and fecal swabs, respectively, submitted for unrelated diagnostic testing. PAstV-4 RNA levels were significantly higher (P = 0.04) in nasal swabs, suggesting a possible atypical respiratory tropism.
对从俄克拉荷马州两个不同商业生产设施中10至21日龄出现不明原因急性呼吸道疾病的猪采集的三份鼻拭子进行宏基因组测序，鉴定出一种新型4型猪星状病毒（PAstV-4）基因型。这些基因组与先前鉴定的PAstV-4分离株的核苷酸序列同一性仅约为75%，而编码衣壳的ORF2与参考菌株的氨基酸序列同一性仅约为53%。针对新型ORF2基因的TaqMan检测法发现，在提交进行无关诊断检测的鼻拭子和粪便拭子中，检出率分别为21%和19%。鼻拭子中的PAstV-4 RNA水平显著更高（P = 0.04），提示可能存在非典型的呼吸道嗜性。
