Saraei Fatemeh, Sadoughi Mehrangiz, Kaka Gholamreza, Sadraie Seyed Homayoon, Foaddodini Mohsen
Department of Biology, Islamic Azad University, North Tehran Branch, Tehran, IR Iran.
Neurosciences Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
Iran Red Crescent Med J. 2016 Mar 12;18(4):e28076. doi: 10.5812/ircmj.28076. eCollection 2016 Apr.
Diazinon is an organophosphate that is broadly used as a pesticide to control insects and environmental pollutions. This toxic material is absorbed via inhalation, contact, or digestion and affects different tissues.
This research was a histomorphometric and immunohistochemical study of the fetal liver of mice after exposure to Diazinon.
Twenty-five pregnant BALB/c mice (25 - 30 gr) were divided into five equal groups in the animal lab of Baqiyatallah University of Medical Sciences, Tehran, Iran. The normal group was without any intervention, and two sham groups received an emulsifier as 0.52 and 5.2 μL/volume (5000 cc in desiccator) and two experimental groups received Diazinon 1.3 and 13μL/volume from the seventh to eighteenth days of pregnancy every other day via forty minutes of inhalation. The pregnant mice were killed on the eighteenth day of gestation and their fetuses were removed and evaluated for fetal growth and liver development. Five fixed fetuses were dehydrated through a series of graded ethanol, embedded in paraffin wax and their whole bodies were sectioned sagittally and stained via the hematoxylin-eosin method. Quantitative computer-assisted morphometric studies were done on the fetal liver tissues occupied by hepatocytes, blood islands, liver sinusoids, and apoptosis.
The mean crown-rump of the fetuses and their mean weight were increased in the experimental group as compared to the sham and normal groups, but the differences were not significant. The mean percentage of the hepatocyte area significantly increased in the experimental group as compared to the sham and control groups (P < 0.0001). However, the mean sinusoid area significantly decreased in the experimental group as compared to the sham and control groups. The mean percentage of the area occupied by apoptotic hepatocytes in the experimental group - 13 μL /volume (8.6143 ± 1.00945) and 1.3 μL /volume (6.1091 ± 0.93093) - significantly increased as compared to the normal and sham groups (P < 0.0001).
Our data showed that inhalation of Diazinon during pregnancy increased the hepatocyte area and hepatocyte apoptosis while it decreased the sinusoid area of the fetal liver.
二嗪农是一种有机磷酸酯，被广泛用作杀虫剂以控制昆虫和环境污染。这种有毒物质可通过吸入、接触或消化被吸收，并影响不同组织。
本研究是对暴露于二嗪农后的小鼠胎儿肝脏进行组织形态计量学和免疫组织化学研究。
在伊朗德黑兰的巴基耶塔拉医科大学动物实验室，将25只怀孕的BALB/c小鼠（体重25 - 30克）分为五组，每组数量相等。正常组不进行任何干预，两个假手术组分别接受0.52和5.2微升/体积（在干燥器中为5000立方厘米）的乳化剂，两个实验组在怀孕的第7天至第18天每隔一天通过40分钟的吸入接受1.3和13微升/体积的二嗪农。在妊娠第18天处死怀孕小鼠，取出胎儿并评估胎儿生长和肝脏发育情况。将5只固定的胎儿通过一系列梯度乙醇脱水，石蜡包埋，然后将其全身矢状切片，并用苏木精-伊红染色法染色。对由肝细胞、血岛、肝血窦和凋亡所占据的胎儿肝脏组织进行定量计算机辅助形态计量学研究。
与假手术组和正常组相比，实验组胎儿的平均顶臀长度及其平均体重有所增加，但差异不显著。与假手术组和对照组相比，实验组肝细胞面积的平均百分比显著增加（P < 0.0001）。然而，与假手术组和对照组相比，实验组肝血窦面积平均显著减小。与正常组和假手术组相比，实验组中凋亡肝细胞所占面积的平均百分比在13微升/体积组（8.6143 ± 1.00945）和1.3微升/体积组（6.1091 ± 0.93093）显著增加（P < 0.0001）。
我们的数据表明，孕期吸入二嗪农会增加胎儿肝脏的肝细胞面积和肝细胞凋亡，同时减小肝血窦面积。
