Yilmaz Nigar, Yilmaz Mustafa, Altuntas Irfan
Department of Biochemistry, Mustafa Kemal University Medical School, Hatay, Turkey.
Toxicol Ind Health. 2012 Feb;28(1):51-7. doi: 10.1177/0748233711404035. Epub 2011 May 4.
Diazinon (DI) is a widely used pesticide in agriculture, resulting in environmental deleterious effects on neural systems. The current study was performed to investigate the effects of treatment with vitamins E plus C on brain toxicity, which is possibly induced by DI. Twenty-one male rats were divided into three groups (n = 7/group) as follows: (1) control group (C); (2) DI-treated group (DI); (3) DI + vitamins E plus C-treated group (DI + Vit). In order to examine lipid peroxidation and antioxidant status in rats, the level of malondialdehyde (MDA), activities of two free radical scavanging enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) have been studied in brain of rat. The results showed that treatment with DI induced significant (p < 0.05) increases in the level of serum MDA in rat brain. The vitamins E plus C combination reduced lipid peroxidation in rat brain. The activity of SOD level was significantly higher in DI + Vit group, compared to the control group. GSH-Px, SOD and CAT values were not significantly different in the DI group than in control. Oxidative stress contributes to DI-induced brain toxicity. Our results suggested that vitamins E plus C combination may have a protective effect on DI-induced brain toxicity.
二嗪农（DI）是一种在农业中广泛使用的杀虫剂，会对神经系统产生环境有害影响。本研究旨在调查维生素E加C联合治疗对可能由DI诱导的脑毒性的影响。将21只雄性大鼠分为三组（每组n = 7）：（1）对照组（C）；（2）DI处理组（DI）；（3）DI +维生素E加C处理组（DI + Vit）。为了检测大鼠的脂质过氧化和抗氧化状态，研究了大鼠脑中丙二醛（MDA）水平、两种自由基清除酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的活性。结果表明，DI处理导致大鼠脑血清MDA水平显著升高（p < 0.05）。维生素E加C联合用药降低了大鼠脑脂质过氧化。与对照组相比，DI + Vit组的SOD水平活性显著更高。DI组的GSH-Px、SOD和CAT值与对照组相比无显著差异。氧化应激导致DI诱导的脑毒性。我们的结果表明，维生素E加C联合用药可能对DI诱导的脑毒性具有保护作用。
