一种基于硫代氨基脲的新型荧光“开启”传感器，用于锌离子（Zn(2+)）识别，具有大斯托克斯位移及其在活细胞成像中的应用
A New Thiosemicarbazone-Based Fluorescence "Turn-on" Sensor for Zn(2+) Recognition with a Large Stokes Shift and its Application in Live Cell Imaging.
作者信息
Tang Lijun, Huang Zhenlong, Zheng Zhuxuan, Zhong Keli, Bian Yanjiang
机构信息
Department of Chemistry, Bohai University, Jinzhou, 121013, China.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian, 116024, China.
出版信息
J Fluoresc. 2016 Sep;26(5):1535-40. doi: 10.1007/s10895-016-1827-y. Epub 2016 Jun 22.
Selective fluorescence turn on Zn(2+) sensor with long-wavelength emission and a large Stokes shift is highly desirable in Zn(2+) sensing area. We reported herein the synthesis and Zn(2+) recognition properties of a new thiosemicarbazone-based fluorescent sensor L. L displays high selectivity and sensitivity toward Zn(2+) over other metal ions in DMSO-H2O (1:1, v/v, HEPES 10 mM, pH = 7.4) solution with a long-wavelength emission at 572 nm and a large Stokes shift of 222 nm. Confocal fluorescence microscopy experiments demonstrate that L is cell-permeable and capable of monitoring intracellular Zn(2+). Graphical Abstract We report a new thiosemicarbazone-based fluorescent sensor (L) for selective recognition of Zn(2+) with a long wavelength emission and a large Stokes shift.
在锌离子传感领域，具有长波长发射和大斯托克斯位移的选择性荧光开启型锌离子传感器是非常理想的。我们在此报道了一种新型硫代卡巴腙基荧光传感器L的合成及其对锌离子的识别特性。在二甲基亚砜 - 水（1:1，v/v，10 mM HEPES，pH = 7.4）溶液中，L对锌离子表现出相对于其他金属离子的高选择性和高灵敏度，发射波长为572 nm，斯托克斯位移为222 nm。共聚焦荧光显微镜实验表明L具有细胞渗透性，能够监测细胞内的锌离子。图形摘要：我们报道了一种新型硫代卡巴腙基荧光传感器（L），用于选择性识别锌离子，具有长波长发射和大斯托克斯位移。
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