一种具有大斯托克斯位移的比率荧光传感器，用于活细胞中锌离子的成像。
A ratiometric fluorescent sensor with a large Stokes shift for imaging zinc ions in living cells.
作者信息
Xue Lin, Liu Chun, Jiang Hua
机构信息
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
出版信息
Chem Commun (Camb). 2009 Mar 7(9):1061-3. doi: 10.1039/b814501f. Epub 2008 Dec 24.
The ratiometric fluorescent sensor based on furoquinoline has been designed and synthesized, and not only exhibits a large Stokes shift of 199 nm in a mixture of C(2)H(5)OH-H(2)O (v/v = 8 ratio 2, 10 mM Tris-HCl, pH = 7.3), but can selectively detect zinc ions in the presence of competing metal ions with a red-shift of 75 nm; moreover, cell stain experiments show that can readily reveal a change in intracellular Zn(2+).
基于呋喹啉的比率荧光传感器已被设计并合成，它不仅在乙醇 - 水混合体系（体积比8:2，10 mM Tris - HCl，pH = 7.3）中表现出199 nm的大斯托克斯位移，而且在存在竞争性金属离子的情况下能够选择性地检测锌离子，红移75 nm；此外，细胞染色实验表明它能够轻松揭示细胞内锌离子的变化。
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