Sivová V, Bera K, Ray B, Nosáľ S, Nosáľová G
Comenius University in Bratislava, Biomedical Center Martin and Department of Pharmacology, Jessenius Faculty of Medicine in Martin, 4C Mala Hora, 03 601, Martin, Slovakia.
Natural Products Laboratory, Department of Chemistry, University of Burdwan, Bardhaman, West Bengal, India.
Adv Exp Med Biol. 2016;935:43-52. doi: 10.1007/5584_2016_30.
In this work we investigated the antitussive activity of the medicinal tree Terminalia arjuna. We used the stem bark for extraction and preparation of water extracted isolate and its two fractions: acetone-soluble (TA-S) and acetone precipitated (TA-P) fraction. The presence of a pectic arabinogalactan was confirmed in TA-P fraction by chromatographic and spectroscopic analysis. The antitussive activity of samples was assessed after oral administration in a dose of 50 mg.kg(-1) in healthy guinea pigs, in which cough was elicited by inhalation of citric acid (0.3 mol/L) in body plethysmograph. The water extracted isolate showed a significant ability to decrease the number of cough efforts by 64.2 %; the antitussive activity on par with that of codeine phosphate. The TA-P fraction showed the antitussive activity of 54.8 %. In contrast, TA-S fraction had only a mild antitussive activity. No changes in in vivo airway resistance were noted. We conclude that arabinogalactan is an essential component of Terminalia arjuna that underlies its antitussive action.
在本研究中，我们调查了药用植物诃子的镇咳活性。我们使用其茎皮进行提取，制备了水提取物及其两个组分：丙酮可溶组分（TA-S）和丙酮沉淀组分（TA-P）。通过色谱和光谱分析证实TA-P组分中存在一种果胶阿拉伯半乳聚糖。在健康豚鼠中，以50 mg·kg⁻¹的剂量口服给药后，评估样品的镇咳活性，豚鼠通过在体体积描记器中吸入柠檬酸（0.3 mol/L）引发咳嗽。水提取物显示出显著的镇咳能力，可使咳嗽次数减少达64.2%；其镇咳活性与磷酸可待因相当。TA-P组分的镇咳活性为54.8%。相比之下，TA-S组分仅具有轻微的镇咳活性。未观察到体内气道阻力的变化。我们得出结论，阿拉伯半乳聚糖是诃子镇咳作用的关键成分。
