Khawas Sadhana, Nosáľová Gabriela, Majee Sujay Kumar, Ghosh Kanika, Raja Washim, Sivová Veronika, Ray Bimalendu
Natural Products Laboratory, Department of Chemistry, The University in Burdwan, West Bengal, India.
Comenius University in Bratislava, Jessenius Faculty of Medicine, Deparment of Pharmacology, Bio-medical Center, Martin, Slovakia.
Int J Biol Macromol. 2017 Jun;99:335-342. doi: 10.1016/j.ijbiomac.2017.02.093. Epub 2017 Feb 28.
Piper nigrum L. fruits are not only a prized spice, but also highly valued therapeutic agent that heals many ailments including asthma, cold and respiratory problems. Herein, we have investigated structural features and in vivo antitussive activity of three fractions isolated from Piper nigrum fruits. The water extract (PN-WE) upon fractionation with EtOH yielded two fractions: a soluble fraction (PN-eSf) and a precipitated (PN-ePf) one. The existence of a pectic polysaccharide with arabinogalactan type II side chains (147kDa) in PN-ePf and piperine in PN-eSf were revealed. Moreover, oligosaccharides providing fine structural details of side chains were generated from PN-ePf and then characterized. The parental water extract (PN-WE) that contained both pectic polysaccharide and piperine, after oral administration (50mgkg body weight) to guinea pigs, showed antitussive activity comparable to codeine phosphate (10mgkg body weight). The EtOH precipitated fraction (PN-ePf) containing pectic polysaccharide showed comparatively higher antitussive activity than EtOH soluble fraction (PN-eSf) that contained piperine, but their potencies are lower than the parental water extract. Significantly, the specific airway smooth muscle reactivity of all three fractions remained unchanged. Finally, pectic polysaccharide-piperine combination in parental extract synergistically enhances antitussive effect in guinea pigs.
胡椒果实不仅是一种珍贵的香料，也是一种备受重视的治疗剂，可治愈多种疾病，包括哮喘、感冒和呼吸道问题。在此，我们研究了从胡椒果实中分离出的三个组分的结构特征和体内镇咳活性。水提取物（PN-WE）用乙醇分级后得到两个组分：一个可溶组分（PN-eSf）和一个沉淀组分（PN-ePf）。揭示了PN-ePf中存在具有II型阿拉伯半乳聚糖侧链的果胶多糖（147kDa）以及PN-eSf中存在胡椒碱。此外，从PN-ePf生成了提供侧链精细结构细节的寡糖，然后对其进行了表征。含有果胶多糖和胡椒碱的亲本水提取物（PN-WE）经口服给予豚鼠（50mg/kg体重）后，显示出与磷酸可待因（10mg/kg体重）相当的镇咳活性。含有果胶多糖的乙醇沉淀组分（PN-ePf）显示出比含有胡椒碱的乙醇可溶组分（PN-eSf）更高的镇咳活性，但其效力低于亲本水提取物。值得注意的是，所有三个组分的特异性气道平滑肌反应性保持不变。最后，亲本提取物中的果胶多糖-胡椒碱组合协同增强了豚鼠的镇咳作用。
