Bonnet J J, Costentin J
U.A. 1170 du C.N.R.S., U.E.R. de Médecine et Pharmacie, Saint Etienne du Rouvray, France.
Life Sci. 1989;44(23):1759-65. doi: 10.1016/0024-3205(89)90563-8.
The development of the specific uptake of dopamine in the rat striatum during the early postnatal period is compared with the ontogenetic changes of the specific binding of (3H)GBR 12783 to the site of uptake inhibition. During maturation, the increase in the specific binding of (3H)GBR 12783 parallels the increase in the specific uptake of dopamine. (3H)GBR 12783 specific binding sites increase in number from day 1 postpartum until 40 days, when they reach the adult level. In 40 day-old rats, the weight of the striatum represents 80% of adult values. The affinity of (3H)GBR 12783 for the inhibition site is similar in membrane preparations obtained from 6 day-old pups and adults; this results in a same ability of the inhibitor to block the specific uptake of dopamine into synaptosomes obtained from pups or adult rats. These data support the hypothesis of the existence of a single molecular entity including both the inhibition site and the carrier itself.
将出生后早期大鼠纹状体中多巴胺特异性摄取的发育过程与(3H)GBR 12783特异性结合到摄取抑制位点的个体发育变化进行比较。在成熟过程中，(3H)GBR 12783特异性结合的增加与多巴胺特异性摄取的增加平行。(3H)GBR 12783特异性结合位点的数量从出生后第1天到40天增加，此时达到成年水平。在40日龄大鼠中，纹状体重量占成年值的80%。从6日龄幼崽和成年大鼠获得的膜制剂中，(3H)GBR 12783对抑制位点的亲和力相似；这导致抑制剂阻断多巴胺特异性摄取到从幼崽或成年大鼠获得的突触体中的能力相同。这些数据支持了存在一种单一分子实体的假设，该实体包括抑制位点和载体本身。
