Clark C G, Cross G A, De Jonckheere J F
Laboratory of Molecular Parasitology, Rockefeller University, New York, NY 10021-6399.
Mol Biochem Parasitol. 1989 May 15;34(3):281-96. doi: 10.1016/0166-6851(89)90057-1.
Ribosomal DNA (rDNA) plasmid restriction maps of 10 strains and rDNA hybridisation patterns of 61 additional strains have been used to assess inter- and intra-specific diversity and phylogenetic relationships in the genus Naegleria. The results obtained by this method largely confirm those of previous studies based on a variety of other criteria. They indicate that very little variation exists within the pathogenic species Naegleria fowleri despite its worldwide distribution and that it is closely related to the nonpathogenic Naegleria lovaniensis. Naegleria gruberi is most likely a polyphyletic grouping and care should be taken when using one strain as a reference point for this species. In addition, the two subspecies of the pathogenic Naegleria australiensis arose separately from within the range of variability encompassed by N. gruberi, as did the species Adelphamoeba galeacystis which should probably be assigned to the genus Naegleria. The species Naegleria jadini and Naegleria andersoni are not closely related to any other in the genus based on their rDNA patterns.
利用10个菌株的核糖体DNA（rDNA）质粒限制图谱以及另外61个菌株的rDNA杂交模式，评估了耐格里属（Naegleria）种间和种内的多样性及系统发育关系。该方法所得结果在很大程度上证实了此前基于各种其他标准开展的研究结果。结果表明，致病种福氏耐格里阿米巴（Naegleria fowleri）尽管分布于全球，但种内变异极小，且与非致病的洛万耐格里阿米巴（Naegleria lovaniensis）密切相关。格氏耐格里阿米巴（Naegleria gruberi）很可能是一个多系类群，以该物种的一个菌株作为参考点时应谨慎。此外，致病的澳大利亚耐格里阿米巴（Naegleria australiensis）的两个亚种是在格氏耐格里阿米巴所涵盖的变异范围内分别产生的，同样，应可能归入耐格里属的囊泡嗜 Adelphamoeba galeacystis 也是如此。基于rDNA模式，贾氏耐格里阿米巴（Naegleria jadini）和安德森耐格里阿米巴（Naegleria andersoni）与该属中的其他任何物种均无密切关系。
