Ozsoy A Z, Nursal A F, Karsli M F, Uysal M, Alici O, Butun I, Tas U, Delibas I B
Department of Obstetrics and Gynecology, School of Medicine, Gaziosmanpasa University, Tokat, Turkey.
Eur Rev Med Pharmacol Sci. 2016 Jun;20(11):2425-34.
Malathion (MLT) is an organophosphate (OP) pesticide widely used in agriculture and for domestic purposes for several years. Intravenous lipid emulsion (ILE) has been reported to reduce toxicity caused by some lipid soluble agents. The aim of this study was to investigate the possible protective effects of ILE treatment on acute malathion toxicity in ovarian tissue of female rats.
Twenty-one adult female Wistar rats (weighted 200-250 g) were divided into three groups; control (corn oil, gavage), MLT (one administration of 100 mg/kg/ by gavage), 20% ILE (one intravenous administration of 3 ml/kg) plus the MLT group. Blood samples were collected for biochemical tests. The ovaries were removed and fixed for histopathological and immunohistochemical analyses. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were investigated in ovarian tissues. Histopathological and immunohistochemical evaluations were performed through scoring ovarian tissue damage and bax/caspase-3 immunoreactivity, respectively.
SOD activity decreased in MLT group compared to the control group in tissue samples (p = 0.012). ILE treatment significantly increased SOD activity in MLT+ILE group compared to MLT group in tissue samples (p = 0.017). MLT treatment increased significantly caspase-3 and bax immunoreactivity while ILE decreased bax and caspase-3 immunoreactivity. However, no significant difference was found for MDA levels and GSH-Px activity in both blood and tissue samples and for histopathological results.
The present study revealed that acute oral MLT administration increased oxidative stress and apoptosis in the rats. ILE treatment partially decreased deleterious effects of MLT. Further controlled animal studies are required to define the role of ILE in acute OP poisonings.
马拉硫磷（MLT）是一种有机磷酸酯（OP）农药，多年来广泛用于农业和家庭用途。据报道，静脉注射脂质乳剂（ILE）可降低某些脂溶性药物引起的毒性。本研究的目的是探讨ILE治疗对雌性大鼠卵巢组织急性马拉硫磷毒性的可能保护作用。
21只成年雌性Wistar大鼠（体重200 - 250克）分为三组；对照组（玉米油，灌胃）、MLT组（单次灌胃100毫克/千克）、20% ILE + MLT组（单次静脉注射3毫升/千克）。采集血样进行生化检测。取出卵巢并固定，用于组织病理学和免疫组织化学分析。检测卵巢组织中的丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）。分别通过对卵巢组织损伤评分和bax/caspase-3免疫反应性进行组织病理学和免疫组织化学评估。
与对照组相比，MLT组组织样本中的SOD活性降低（p = 0.012）。与MLT组相比，ILE治疗使MLT + ILE组组织样本中的SOD活性显著增加（p = 0.017）。MLT治疗显著增加了caspase-3和bax免疫反应性，而ILE降低了bax和caspase-3免疫反应性。然而，血液和组织样本中的MDA水平和GSH-Px活性以及组织病理学结果均未发现显著差异。
本研究表明，急性口服MLT可增加大鼠的氧化应激和细胞凋亡。ILE治疗部分降低了MLT的有害作用。需要进一步进行对照动物研究来确定ILE在急性OP中毒中的作用。
