Esen M, Uysal M
Bratisl Lek Listy. 2018;119(6):373-378. doi: 10.4149/BLL_2018_069.
Organophosphates including malathion can be ingested, inhaled, or absorbed through the skin, but it may be a maximum of acute toxicity when administered by oral intake. Intravenous lipid emulsion (ILE) treatment is used as a new treatment method in cases of systemic toxicity caused by local anesthetics. This study was aimed to examine the potential treatment effect of intravenous lipid emulsion on rat liver tissue in the toxicity of malathion.
Twenty-one Wistar albino rats were randomly divided into three equal groups. The groups were organized as Group I (control), Group II (malathion) and Group III (malathion + lipid emulsion treatment). Liver tissues were examined histologically, and immunohistochemical analysis was performed to determine the bax, bcl-2, and caspase-3 expression levels.
A decrease of PAS positive staining cells, and an increase of liver enzymes, formation of degenerative changes and apoptotic cell deaths occurred in the malathion group. Additionally, a decrease of apoptosis and hepatic parenchymal damage was observed in the malathion + lipid emulsion treatment group.
The findings from our study suggested that lipid emulsion treatment had a protective efficacy on the malathion induced liver toxicity (Fig. 5, Ref. 30).
包括马拉硫磷在内的有机磷酸酯可通过摄入、吸入或经皮肤吸收，但经口服给药时可能具有最大急性毒性。静脉注射脂质乳剂（ILE）治疗被用作局部麻醉药引起的全身毒性病例的一种新治疗方法。本研究旨在探讨静脉注射脂质乳剂对马拉硫磷中毒大鼠肝脏组织的潜在治疗效果。
将21只Wistar白化大鼠随机分为三组，每组数量相等。分组如下：第一组（对照组）、第二组（马拉硫磷组）和第三组（马拉硫磷+脂质乳剂治疗组）。对肝脏组织进行组织学检查，并进行免疫组织化学分析以确定bax、bcl-2和caspase-3的表达水平。
马拉硫磷组出现PAS阳性染色细胞减少、肝酶升高、退行性改变形成和凋亡性细胞死亡。此外，在马拉硫磷+脂质乳剂治疗组中观察到凋亡和肝实质损伤减少。
我们的研究结果表明，脂质乳剂治疗对马拉硫磷诱导的肝毒性具有保护作用（图5，参考文献30）。
