Li Qi, Gu Wenbo, Ma Xuan, Liu Yuxin, Jiang Lidan, Feng Rennan, Liu Liyan
Department of Nutrition and Food Hygiene, National Key Discipline, Public Health College, Harbin Medical University, Harbin 150086, China.
Nutrients. 2016 Jun 21;8(6):379. doi: 10.3390/nu8060379.
Hyperlipidemia (HLP) is characterized by a disturbance in lipid metabolism and is a primary risk factor for the development of insulin resistance (IR) and a well-established risk factor for cardiovascular disease and atherosclerosis. The aim of this work was to investigate the changes in postprandial amino acid and biogenic amine profiles provoked by an oral glucose tolerance test (OGTT) in HLP patients using targeted metabolomics. We used ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry to analyze the serum amino acid and biogenic amine profiles of 35 control and 35 HLP subjects during an OGTT. The amino acid and biogenic amine profiles from 30 HLP subjects were detected as independent samples to validate the changes in the metabolites. There were differences in the amino acid and biogenic amine profiles between the HLP individuals and the healthy controls at baseline and after the OGTT. The per cent changes of 13 metabolites from fasting to the 2 h samples during the OGTT in the HLP patients were significantly different from those of the healthy controls. The lipid parameters were associated with the changes in valine, isoleucine, creatine, creatinine, dimethylglycine, asparagine, serine, and tyrosine (all p < 0.05) during the OGTT in the HLP group. The postprandial changes in isoleucine and γ-aminobutyric acid (GABA) during the OGTT were positively associated with the homeostasis model assessment of insulin resistance (HOMA-IR; all p < 0.05) in the HLP group. Elevated oxidative stress and disordered energy metabolism during OGTTs are important characteristics of metabolic perturbations in HLP. Our findings offer new insights into the complex physiological regulation of metabolism during the OGTT in HLP.
高脂血症（HLP）的特征是脂质代谢紊乱，是胰岛素抵抗（IR）发生的主要危险因素，也是心血管疾病和动脉粥样硬化公认的危险因素。本研究的目的是使用靶向代谢组学研究HLP患者口服葡萄糖耐量试验（OGTT）引发的餐后氨基酸和生物胺谱的变化。我们使用超高效液相色谱-三重四极杆质谱法分析了35名对照者和35名HLP受试者在OGTT期间的血清氨基酸和生物胺谱。将30名HLP受试者的氨基酸和生物胺谱作为独立样本进行检测，以验证代谢物的变化。在基线和OGTT后，HLP个体与健康对照者的氨基酸和生物胺谱存在差异。HLP患者在OGTT期间从空腹到2小时样本的13种代谢物的百分比变化与健康对照者有显著差异。在HLP组OGTT期间，血脂参数与缬氨酸、异亮氨酸、肌酸、肌酐、二甲基甘氨酸、天冬酰胺、丝氨酸和酪氨酸的变化相关（所有p<0.05）。在HLP组OGTT期间，异亮氨酸和γ-氨基丁酸（GABA）的餐后变化与胰岛素抵抗的稳态模型评估（HOMA-IR；所有p<0.05）呈正相关。OGTT期间氧化应激升高和能量代谢紊乱是HLP代谢紊乱的重要特征。我们的研究结果为HLP患者OGTT期间代谢的复杂生理调节提供了新的见解。
