Mao Jie, Sun Xing, Cheng Jian-Hua, Shi Yong-Jie, Wang Xin-Zheng, Qin Jun-Jie, Sang Zhi-Hong, He Kun, Xia Qing
State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, 27 Tai-Ping Road, Beijing 100850, China.
State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, 27 Tai-Ping Road, Beijing 100850, China.
Food Chem Toxicol. 2016 Sep;95:1-11. doi: 10.1016/j.fct.2016.06.015. Epub 2016 Jun 20.
A 52-week feeding study in cynomolgus macaques was carried out to evaluate the safety of Bt rice Huahui 1 (HH1), a transgenic rice line expressing Cry1Ab/1Ac protein. Monkeys were fed a diet with 20% or 60% HH1 rice, 20% or 60% parental rice (Minghui 63, MH63), normal diet, normal diet spiked with purified recombinant Cry1Ab/1Ac fusion protein or bovine serum albumin (BSA) respectively. During the feeding trail, clinical observations were conducted daily, and multiple parameters, including body weight, body temperature, electrocardiogram, hematology, blood biochemistry, serum metabolome and gut microbiome were examined at regular intervals. Upon sacrifice, the organs were weighted, and the macroscopic, microscopic and electron microscopic examinations were performed. The results show no adverse or toxic effects of Bt rice HH1 or Cry1Ab/1Ac fusion protein on monkeys. Therefore, the present 52-week primate feeding study suggests that the transgenic rice containing Cry 1Ab/1Ac is equivalent to its parental rice line MH63.
开展了一项为期52周的食蟹猴喂养研究，以评估表达Cry1Ab/1Ac蛋白的转基因水稻华恢1号（HH1）的安全性。猴子分别喂食含有20%或60% HH1水稻、20%或60%亲本水稻（明恢63，MH63）的日粮、正常日粮、添加了纯化重组Cry1Ab/1Ac融合蛋白的正常日粮或牛血清白蛋白（BSA）。在喂养试验期间，每天进行临床观察，并定期检查包括体重、体温、心电图、血液学、血液生化、血清代谢组和肠道微生物群在内的多个参数。处死后，对器官进行称重，并进行大体、显微镜和电子显微镜检查。结果表明，Bt水稻HH1或Cry1Ab/1Ac融合蛋白对猴子没有不良或毒性作用。因此，目前这项为期52周的灵长类动物喂养研究表明，含有Cry 1Ab/1Ac的转基因水稻与其亲本水稻品系MH63相当。
