Kielak Anna M, Cipriano Matheus A P, Kuramae Eiko E
Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P.O. Box 50, Droevendaalsesteeg 10, 6708 PB, Wageningen, The Netherlands.
Arch Microbiol. 2016 Dec;198(10):987-993. doi: 10.1007/s00203-016-1260-2. Epub 2016 Jun 23.
Acidobacteria is one of the most abundant phyla in soils and has been detected in rhizosphere mainly based on cultivation-independent approaches such as 16S rRNA gene survey. Although putative interaction of Acidobacteria with plants was suggested, so far no plant-bacterial interactions were shown. Therefore, we performed several in vitro tests to evaluate Acidobacteria-plant interactions and the possible mechanisms involved in such interaction. We observed that Arabidopsis thaliana inoculated with three strains belonging to Acidobacteria subdivision 1 showed increase in biomass of roots and shoots as well as morphological changes in root system. Our results indicate that the plant hormone indole-3-acetic acid production and iron acquisition are plausibly involved in the plant and Acidobacteria interactions. Here, we confirm for the first time that Acidobacteria can actively interact with plants and act as plant growth-promoting bacteria. In addition, we show that Acidobacteria strains produce exopolysaccharide which supports the adhesion of bacteria to the root surfaces.
酸杆菌门是土壤中最丰富的菌门之一，主要通过诸如16S rRNA基因调查等非培养方法在根际中检测到。尽管有人提出酸杆菌与植物之间可能存在相互作用，但迄今为止尚未发现植物与细菌之间的相互作用。因此，我们进行了多项体外试验，以评估酸杆菌与植物的相互作用以及这种相互作用涉及的可能机制。我们观察到，接种了属于酸杆菌门第1亚群的三株菌株的拟南芥，其根和地上部分的生物量增加，根系形态也发生了变化。我们的结果表明，植物激素吲哚-3-乙酸的产生和铁的获取可能参与了植物与酸杆菌的相互作用。在这里，我们首次证实酸杆菌可以与植物积极相互作用，并作为植物促生细菌发挥作用。此外，我们表明酸杆菌菌株产生胞外多糖，这有助于细菌粘附到根表面。
